نتایج جستجو برای: microspectroscopy
تعداد نتایج: 1198 فیلتر نتایج به سال:
Candida antarctica Lipase B (CALB) was covalently immobilized onto epoxy-activated macroporous poly(methyl methacrylate) Amberzyme beads (235 microm particle size, 220 A pore size) and nanoparticles (nanoPSG, diameter 68 nm) with a poly(glycidyl methacrylate) outer region. Amberzyme beads allowed CALB loading up to 0.16 g of enzyme per gram of support. IR microspectroscopy generated images of A...
Human hair is typically 50-100 μ in diameter, previous transmission and ATR studies of its cross section1,2 reveal three major identifiable regions. The core, or medulla, typically 5-10 μ in diameter, is surrounded by the cortex, making up the bulk of the hair and finally an outer layer or cuticle, typically less than 5 μ in thickness. Biochemical compositional studies may be performed by exami...
Recent developments in biomedical vibrational spectroscopy now permit the non-invasive imaging of cells and tissues within both the laboratory and clinical settings. The rapid nature and diagnostic potential of both Raman and FTIR (Fourier-transform IR) spectroscopy have resulted in their widespread application to a number of biological fields including fundamental cell biology, medical imaging...
PURPOSE To establish a tear analytical model by using Raman microspectroscopy to assess ocular surface diseases associated with infectious pathogens. METHODS The authors applied confocal Raman microspectroscopy based on the drop-coating deposition method on Ti/Au-coated glass slides to obtain sample spectra from different types of tears, including simplified synthetic tears (SSTs), SSTs mixed...
In this study, confocal Raman microspectroscopy was used to detect lipids in microalgae rapidly and non-destructively. Microalgae cells were cultured under nitrogen deficiency. The accumulation of lipids in Scenedesmus obliquus was observed by Nile red staining, and the total amount of lipids accumulated in the cells was measured by gravimetric method. The signals from different microalgae cell...
Microbial communities are essential to the function of virtually all ecosystems and eukaryotes, including humans. However, it is still a major challenge to identify microbial cells active under natural conditions in complex systems. In this study, we developed a new method to identify and sort active microbes on the single-cell level in complex samples using stable isotope probing with heavy wa...
BACKGROUND AND AIMS Rhinanthus minor is a root hemiparasitic plant that attacks a wide range of host species which are severely damaged by the parasite. Rhinanthus minor also attempts unsuccessfully to form connections to a range of non-hosts which in contrast are not damaged by the parasite; however, the underlying physiological basis of these differences is not fully understood. METHODS Bio...
The aim of the paper is to study phase transformations in solid tissues of the human teeth during the development of fissure caries by Raman and fluorescence microspectroscopy. The study of the areas with fissure caries confirmed the assumption of the formation of a weak interaction between phosphate apatite enamel and organic acids (products of microorganisms). The experimental results obtaine...
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