BACKGROUND The enzyme-linked immunosorbent assay (ELISA) has been used for diagnosing medical and plant pathologies. In addition, it is used for quality-control evaluations in various industries. The ELISA is the simplest method for obtaining excellent results; however, it is time consuming because the immunoreagents interact only on the contact surfaces. Antibody-labeled magnetic particles can...

The fluorescent compound 4-methylumbelliferone (4MU) can be used to detect uridine diphosphate glucuronosyl transferase activity by observing the fall in fluorescence as the compound is converted to 4-methylumbelliferone glucuronide. A microplate assay has been developed that has improved sensitivity and is faster and cheaper than the historical extraction method. Activity is detectable with ap...

This case series describes an alternative approach for removing ectopically erupted tooth in the maxillary sinus using improved endoscopic known as modified endoscopic-assisted surgery (MESS). We reviewed medical charts and radiographs of patients who underwent surgical removal ectopic MESS. The technique consisted creating a bony window wall with pre-adapted microplate, which was repositioned ...

A modified ELISA procedure is described which permits selective detection of monoclonal antibodies reactive with the native antigen. The essence of the method is the use of immobilization methods that do not result in denaturation of the antigen during adsorption to the microtiter plates. This was accomplished by using polyclonal antibodies, adsorbed to the wells of the microtiter plate, as the...

We modified the phosphocellulose binding assay for aminoglycoside-modifying enzymes (AMEs) by use of microdilution plates and a multichannel micropipette. Batteries of aminoglycoside substrates for screening organisms for the presence of AMEs as well as for subclassifying enzymes were prepared and stored in microdilution plates. When tested in parallel with the conventional tube reaction assay,...

this study investigated the effect of reciprocal teaching (rt) on efl learners’ reading comprehension. fifty intermediate learners participated in the study and were sampled as the experimental (n = 25) and control groups (n = 25). participants were male and ranged in age from 15 to 16. the reading section of key english test (ket, 2010) was used as the pretest to assess the participants’ entry...

We present a reduced-order model for electrically actuated microplate-based MEMS. The model accounts for the electric force nonlinearity and the mid-plane stretching of the plate. The linear undamped vibration modes are found numerically using the hierarchical finite-element method. These mode shapes are used in a Galerkin approximation to reduce the partialdifferential equations of motion and ...

Lactic acid bacteria (LAB) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (GRAS) by the FDA for use as food ingredients. In addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. In this study we examined the inhibition of Listeria monocytogenes 39-2 by neutralized and non-n...

The green fluorescent protein (GFP) gene offers many advantages as a viability reporter for high-throughput antimicrobial drug screening. However, screening for antituberculosis compounds by using GFP driven by the heat shock promoter, hsp60, has been of limited utility due to the low signal-to-noise ratio. Therefore, an alternative promoter was evaluated for its enhanced fluorescence during mi...