Five cannulated Holstein steers (302 +/- 23 kg) were used in a 4 x 4 Latin square design experiment with extra observations to evaluate the effect of supplemental protein source on postruminal flow and intestinal disappearance of N and amino acids (AA). Diets were formulated to contain 12.5% CP using cracked corn (70%), ground corn cobs (15%), and supplement (15%). Except for an all-urea supple...

Despite the growing interest to explore untapped microbial gene and protein diversity, no single platform has been able to acquire both gene and protein information from just a few cells. We present a microfluidic system that simultaneously performs on-chip capillary electrophoresis for protein analysis and whole genome amplification (WGA), and we demonstrate this by doing both for the same coh...

This experiment was conducted to determine the possibility of replacing part of the barley grain by hydroponic barley fodder (HBF), and evaluating its effect on digestibility, rumen parameters, microbial protein synthesis and blood parameters in sheep. Four male Kermani sheep, 18-month-old (34.7 ± 1 kg live weight) were randomly assigned to a change over 4 × 4 design and fed the experimental di...

Although amino acids (AA) synthesized by enteric microbiota in the upper gut of nonruminants can be absorbed, they do not necessarily make a net contribution to the host's AA supply. That depends on whether protein or nonprotein nitrogen sources are used for microbial protein production. We determined the contributions of urea, endogenous protein (EP), and dietary protein (DP) to microbial vali...

BACKGROUND Natural microbial communities are extremely complex and dynamic systems in terms of their population structure and functions. However, little is known about the in situ functions of the microbial communities. RESULTS This study describes the application of proteomic approaches (metaproteomics) to observe expressed protein profiles of natural microbial communities (metaproteomes). T...

The nutritive value of pistachio epicarp (PE) was evaluated by in situ and in vitro techniques. Chemical analysis indicated that PE was high in crude protein (11.30%) and low in neutral detergent fiber (26.20%). Total phenols, total tannins, condensed tannins and hydrolysable tannins contents in PE were 8.29%, 4.48%, 0.49% and 3.79%, respectively. Ruminal dry matter and crude ...

The main objective of the thesis was to study AA supply of dairy cows fed grass silage based diets with specific emphasis on the impact of changing individual and total AA supplies arising from individual AA supplementations or changes in the diet on milk production and AA utilisation. Mammary AA metabolism was also studied to broaden the understanding of the mechanisms underlying altered lacta...

Many mammals are herbivores, and most herbivorous mammals live on a diet that makes cellulose digestion essential. The ruminants, which include some of our most important domestic meatand milk-producing animals cattle, sheep and goats have a specialized digestive tract which is highly adapted and has evolved to digest highly fibrous plant materials which nonruminants are unable to utilize. In r...

Molecular mimicry refers to structural homologies between a self-protein and a microbial protein. A major epitope of myelin basic protein (MBP), p87-99 (VHFFKNIVTPRTP), induces experimental autoimmune encephalomyelitis (EAE). VHFFK contains the major residues for binding of this self-molecule to T cell receptor (TCR) and to the major histocompatibility complex. Peptides from papilloma virus str...