The kinetics of a very large NAD-dependent glutamate dehydrogenase from Janthinobacterium lividum showed positive cooperativity toward alpha-ketoglutarate and NADH, and the Michaelis-Menten type toward ammonium chloride in the absence of the catalytic activator, L-aspartate. An increase in the maximum activity accompanied the decrease in the S(0.5) values for alpha-ketoglutarate and NADH with t...

This review traces the history and logical progression of methods for quantitative analysis of enzyme kinetics from the 1913 Michaelis and Menten paper to the application of modern computational methods today. Following a brief review of methods for fitting steady state kinetic data, modern methods are highlighted for fitting full progress curve kinetics based upon numerical integration of rate...

The validity of the Michaelis-Menten-Briggs-Haldane approximation for single enzyme reactions has recently been improved by the formalism of the total quasi-steady-state approximation. This approach is here extended to fully competitive systems, and a criterion for its validity is provided. We show that it extends the Michaelis-Menten-Briggs-Haldane approximation for such systems for a wide ran...

Abstract Shewanella xiamenensis G5-03 was observed to decolorize the azo dye Congo red in synthetic wastewater. The influence of some factors on decolorization efficiency evaluated. optimal conditions were temperature 30-35 °C, pH 10.0, incubation time 10 h, and static condition. kinetic fitted Michaelis–Menten model (Vmax = 111.11 mg L-1 h-1 Km 448.3 L-1). bacterium also able degrade benzidine...

the oxidation of n-pentanol by tetramethylammonium fluorochromate in acidic solution wasstudied using spectrophotometric technique. the reaction was arranged to be under pseudo firstorderconditions respect to the oxidant. a michaelis-menten type kinetic was observed respect tothe substrate. the reaction is catalyzed by hydrogen ions. dependences of the reaction rates ontemperature and different...

A key step towards a chemical picture of enzyme catalysis was taken in 1913, when Leonor Michaelis and Maud Menten published their studies of sucrose hydrolysis by invertase. Based on a novel experimental design and a mathematical model, their work offered a quantitative view of biochemical kinetics well before the protein nature of enzymes was established and complexes with substrates could be...

In this paper, a spatial tri-trophic food chain model with ratio-dependent Michaelis-Menten type functional response under homogeneous Neumann boundary conditions is studied. Conditions for Hopf and Turing bifurcation are derived. Sufficient conditions for the emergence of spatial patterns are obtained. The results of numerical simulations reveal the formation of labyrinth patterns and the coex...

ER aminopeptidase 1 (ERAP1) customizes antigenic peptide precursors for MHC class I presentation and edits the antigenic peptide repertoire. Coding single nucleotide polymorphisms (SNPs) in ERAP1 were recently linked with predisposition to autoimmune disease, suggesting a link between pathogenesis of autoimmunity and ERAP1-mediated Ag processing. To investigate this possibility, we analyzed the...

This article deals with a 2 ? reaction-diffusion-taxis model consisting of Michaelis-Menten functional response predator-prey system. The critical section this is that temporal-spatial evolution the predators? velocity depends largely on gradient prey. But beyond that, system also inscribes prey-taxis mechanism an immediate movement predator u in to change prey v (which leads collection u). By ...