Figure S 1: Chemical structure of BODIPY-cholesterol (BChol) and dehydroergosterol (DHE). A, structure of BODIPY-cholesterol with the fluorophore (green, with light green underlay to highlight the fluorescent group) at carbon 24 of the sterol side chain. B, structure of DHE. Differences to cholesterol are indicated in green and red; i.e., an extra methyl group and double bond in the alkyl side ...

Specialized adaptations for application of Goldner's Masson trichrome stain to plastic embedded undecalcified bone specimens are presented. This stain can be used successfully on methyl-glycol methacrylate, glycol methacrylate and Spurr embedded bones. The stain affords the advantage of good cellular staining due to the hematoxylin component with concomitant sharp discrimination of mature bone ...

Three compounds containing moieties rarely encountered in nature, viz., 3-nonyloxirane-2 carboxylic acid methyl ester (1), 2-chlorododec-2-en-1-ol (2), and 2-chlorododec-2,11-dien-1-ol (3), were isolated from the red alga Gracilaria verrucosa, and their structures were determined by spectroscopic methods.

The colon group of bacteria may be characterized as nonsporing Gram-negative bacilli which ferment lactose, producing acid and gas, and which can grow aerobically. Although many bacteriologists recognize the genera Escherichia and Aerobacter, the genus Citrobacter has not been generally accepted. In 1923, Koser showed that strains of the genus Aerobacter utilized citric acid as a sole source of...

(Trimethylsilyl)methyl groups incorporated at 9- and 9,10-positions of anthracenes and at 1,3,6,8-positions of pyrenes were found to orient perpendicular to the aromatic frameworks in their crystals and induce red-shift of UV absorption and fluorescence spectra and enhancement of fluorescence quantum yields as compared with the parent hydrocarbons.

A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale green. The method was significantly better than Giemsa staining and comparable to...

The abbreviations used are: Cygb, cytoglobin; FHb, flavohemoglobin; GCS, globin-coupled sensor; Hb, hemoglobin; HBL, hexagonal bilayer; Mb, myoglobin; MCP, methyl accepting chemotaxis protein; Ngb, neuroglobin; NS, nonsymbiotic; RBC, red blood cell; SD, single domain; SDF, SD FHb-like; SDS, http://www.jbc.org/cgi/doi/10.1074/jbc.R700029200 The latest version is at JBC Papers in Press. Published...

Ellagitannins from red raspberries (Rubus idaeus) and cloudberries (Rubus chamaemorus) were isolated by using column chromatography and preparative HPLC. The berry phenolic isolates consisted of 80% (cloudberry) and of 60% (raspberry) of ellagitannins, with raspberries also containing anthocyanins. The main ellagitannins of both raspberries and cloudberries were identified by ESI-MS to consist ...

Azo dyes represent the world’s largest and most diverse group of synthetic dyes with uses spanning almost every industry (Pandey et al. 2007). Their significance to public health is great as these globally produced dyes have been proven to be carcinogenic upon biodegrading into their aromatic amines (Harmse 2013). But aside from their carcinogenic properties, azo dyes have another dangerous eff...