Many workers have demonstrated that extrahepatic tissues utilize ketone bodies in the absence of insulin (1). However, it has been postulated that a decrease in peripheral utilization of ketone bodies is a contributory factor in diabetic ketosis (2). No careful comparison of peripheral utilization by muscle from diabetic and nondiabetic preparations has been reported using modern techniques. Th...

acetoacetate level is markedly raised it does not contribute to any increase in the third spot. The absence of any detectable acetoacetate spot is probably due to decarboxylation, both during the extraction process and during the run on the paper, and it was noticed that an acetone 2:4-dinitrophenylhydrazone spot was regularly seen on the chromatograms from the alloxan-treated animals. In view ...

In this sensitive, reproducible method for determination of D-3-hydroxybutyrate (3-OHB) in plasma, it is converted to acetone by use of 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30)/lactate dehydrogenase (EC 1.1.1.27) coupled with acetoacetate decarboxylase (EC 4.1.1.4). The resulting acetone is detected by head-space gas chromatography. The lowest concentration of 3-OHB detectable in plasma wa...

1. The colorimetric method for estimation of acetoacetate by reaction with diazotizedp-nitroaniline has been adapted for measurements of oxaloacetate in amounts of 0 003-0 03 Htmole. 2. The steady-state level of oxaloacetate in the livers of normal and fasted rats was found to be about 4 umoles/100 g. dry wt. of tissue. The level was essentially unchanged in starvation when the level of acetoac...

One pot syntheses of furan, thiophene, and pyrrole were accomplished by oxidative deacetylation using Mn(III)/Co(II) catalysts and the Paal-Knorr reaction from 1,5-dicarbonyl compounds, which are prepared from the conjugate addition of ethyl acetoacetate to α,β-unsaturated carbonyl compounds. The oxidative deacetylation and reductive cyclization of β-ketoesters derived from ethyl acetoacetate a...

Type-1 diabetic patients experience hyperketonemia caused by an increase in fatty acid metabolism. Thus, the aim of this study was to measure the effect of ketone bodies as suppressors of oxidizing species produced by stimulated neutrophils. Both acetoacetate and 3-hydroxybutyrate have suppressive effect on the respiratory burst measured by luminol-enhanced chemiluminescence. Through measuremen...

A series of experiments was performed during the induction of starvation ketosis and in the acute reversal of the ketotic state. In contrast to the predictions of two widely held theories of ketogenesis, control of acetoacetate production by the liver appeared to be unrelated to changes in fatty acid mobilization from the periphery, fatty acid oxidation, fatty acid synthesis, or the acetyl coen...

Many workers have demonstrated that extrahepatic tissues utilize ketone bodies in the absence of insulin (1). However, it has been postulated that a decrease in peripheral utilization of ketone bodies is a contributory factor in diabetic ketosis (2). No careful comparison of peripheral utilization by muscle from diabetic and nondiabetic preparations has been reported using modern techniques. Th...

We have investigated the application of multipoint kinetic curve-fitting methods to the determination of an analyte in the presence of a single interferent. Our model system for the analyte-interferent was creatinine-acetoacetate as determined with the kinetic Jaffé method. We examined the utility of the following multipoint approaches: simultaneous equations, multivariable linear regression, a...