We report characterization of a methicillin-susceptible, vancomycin-resistant bloodstream isolate of Staphylococcus aureus recovered from a patient in Brazil. Emergence of vancomycin resistance in methicillin-susceptible S. aureus would indicate that this resistance trait might be poised to disseminate more rapidly among S. aureus and represents a major public health threat.

Methicillin resistance (Mecr) was transduced into a methicillin-susceptible variant of the Mecr donor Staphylococcus epidermidis BS10. UV irradiation of phage stimulated Mecr transduction frequency. If loss of Cd and Hg ion resistance occurred in this recipient, or if the three markers Mecr, Cdr, and Hgr were co-eliminated from the donor, neither strain acted as a recipient for Mecr.

conclusions since it is highly important to rapidly detect mrsa isolates, especially in nosocomial infections, phenotypic methods may certainly be useful for this purpose. resistance to methicillin in s. aureus shows a substantially increased ability in turanose metabolism. it is concluded that fermentation of turanose at 0.7% dilution could be a rapid detection method for primary screening of ...

BACKGROUND Screening for methicillin-resistant Staphylococcus aureus (MRSA) is intended to reduce nosocomial spread by identifying patients colonized by MRSA. Given the widespread use of this screening, we evaluated its potential clinical utility in predicting the resistance of clinical isolates of S. aureus. METHODS We conducted a 2-year retrospective cohort study that included patients with...

background and objectives: staphylococcus aureus is a versatile organism causing mild to life threatening infections. the major threat of this organism is its multidrug resistance. the present study was carried out to investigate in - vitro activity of conventional antibiotics routinely prescribed for methicillin resistant s. aureus (mrsa) and methicillin sensitive s. aureus (mssa) infections i...

Background: Methicillin-Resistant Staphylococcus aureus (MRSA) has been an important cause of bone infection since the 1940s. Current guidelines recommend targeted antibiotic use for osteomyelitis treatment informed by microbial sensitivity patterns. However, in settings without microbiology facilities, empirical antibiotic use is common. Unrecognized antibiotic resistance potentiates persisten...

The commonly isolated organisms, Staphylococcus aureus, Escherichia coli, Klebsiella species, Pseudomonas aeruginosa, Acinetobacter species, Proteus species and Enterobacter species from clinical material other than blood, cerebrospinal fluid and stool, were analysed for their incidence and increasing trends of resistance to the commonly used antimicrobials. Staphylococcus aureus was tested aga...

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important causes hospital infections worldwide. S. tends to be resistant multiple antibiotics. High-level resistance antibiotics caused by mecA gene, which encodes an alternative penicillin-binding protein, PBP 2a. The present study was aimed detect in potential isolates clinical wastewater. Three wastewater samples were coll...