CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of an...

Sister chromatid separation in anaphase depends on the removal of cohesin complexes from chromosomes. In vertebrates, the bulk of cohesin is already removed from chromosome arms during prophase and prometaphase, whereas cohesin remains at centromeres until metaphase, when cohesin is cleaved by the protease separase. In unperturbed mitoses, arm cohesion nevertheless persists throughout metaphase...

Previous studies of the kinetochore in mammalian systems have demonstrated that this structure undergoes reorganizations after microtubule attachment or in response to activation of the spindle checkpoint. Here, we show that the Caenorhabditis elegans kinetochore displays analogous rearrangements at prometaphase, when microtubule/chromosome interactions are being established, and after exposure...

We have designed experiments that distinguish centrosomal , nuclear, and cytoplasmic contributions to the assembly of the mitotic spindle. Mammalian centrosomes acting as microtubule-organizing centers were assayed by injection into Xenopus eggs either in a metaphase or an interphase state. Injection of partially purified centrosomes into interphase eggs induced the formation of extensive aster...

We have identified a novel human centromere-associated protein by preparing monoclonal antibodies against a fraction of HeLa chromosome scaffold proteins enriched for centromere/kinetochore components. One monoclonal antibody (mAb177) specifically stains the centromere region of mitotic human chromosomes and binds to a novel, approximately 250-300 kd chromosome scaffold associated protein named...

Cytostatic factor (CSF) arrests vertebrate eggs in metaphase of meiosis II through several pathways that inhibit activation of the anaphase-promoting complex/cyclosome (APC/C). In Xenopus, the Mos-MEK1-MAPK-p90(Rsk) cascade utilizes spindle-assembly-checkpoint components to effect metaphase arrest. Another pathway involves cyclin E-Cdk2, and sustained cyclin E-Cdk2 activity in egg extracts caus...

Regardless of rich biodiversity, cytogenetic reports on Pentatomidae refer to less than 400 species. This paper reports, for the first time, course of meiosis of three species of subfamily Pentatominae viz., Tolumnia antennata Distant, Cahara jugatoria (Lethierry) and Cahara confusa (Distant). Species-specific variations observed with respect to association pattern of X and Y during prophase, a...

Introduction Various structural studies have demonstrated that the chromosomal architecture of the nucleus is highly ordered and suggest that it might be held in place by some sort of nuclear scaffold. This organization must permit dynamic movements of chromatin during activities such as enhancer looping, recombination and chromosome condensation. The mechanisms whereby nuclear order and chroma...

a 56-year-old man with a two year history of chronic stable angina underwent elective coronary artery bypass grafting (cabg) due to angiographic report of three vessel disease and tight stenosis at proximal part of left anterior descending artery (lad). while harvesting of radial artery (ra), the distal half of radial artery was found to bifurcate to two parallel branches with equal size. we us...