ObjectiveTo determine whether the ooplasm granulation patterns of donor oocytes, like those oocytes from poor-prognosis patients, are predictive in vitro fertilization (IVF) outcomes.DesignRetrospective cohort study.SettingAcademically affiliated private clinical infertility and research center.Patient(s)770 fresh 381 vitrified-thawed metaphase II young donors (aged 21.0–34.6 years) used for IV...

in traditional medicine, mentha spicata is widely used for many diseases, especially in  digestive system. it has anti-androgenic effects thus can be used by women experiencing polycystic ovary syndrome (pcos). also, it has shown to possess antioxidant properties. although beneficial, studies have shown its detrimental effect on some tissues. the present study was carried out to determine the e...

Background: Intracytoplasmic Sperm Injection (ICSI) is one of the methods of assisted reproductive technology that aims to successfully treat male factor infertility in humans and domestic animals. The technique involves mechanically injecting a single sperm or sperm nucleus with the help of a micromanipulator into the cytoplasm of a mature metaphase II oocyte. Results: In domestic animals, ICS...

To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes, and 14...

The release of a mature healthy egg for fertilization is the center of the entire reproductive process. From the time of embryonic development till fertilization, the oocyte undergoes several stop-and-go periods. In most animals, oocytes are held in meiotic arrest in prophase I prior to ovulation. The ovulatory luteinizing hormone (LH) surge promotes the resumption of meiosis of the arrested oo...

BACKGROUND An inverse relationship between oocyte efficiency and ovarian response was reported in conventional IVF. The purpose of this study was to report metaphase II (MII) oocyte efficiency according to oocyte yield in minimal/mild stimulation IVF (mIVF) and to assess whether oocyte yield affects live birth rate (LBR). METHODS Infertile women (n = 264) aged < 39 years old with normal ovari...

rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. in this study we investigated the effects of supplementation of cysteamine on the rate of in vitro maturation of oocytes in two different media. germinal vesicle oocytes were collected from mouse ovary and cultured in two media (tcm199 and meme) with 0, 50, 100, 200, 500 µm/ml cysteamine. number...

Mammalian oocyte meiotic divisions are highly asymmetric and produce a large haploid gamete 2 small polar bodies. This relies on the ability of cell to break symmetry position its spindle close cortex before anaphase occurs. In metaphase II–arrested mouse oocytes, is actively maintained parallel cortex, until fertilization triggers sister chromatid segregation rotation spindle. The latter must ...