نتایج جستجو برای: metalloenzyme

تعداد نتایج: 521  

Journal: :Kataliz v promyšlennosti 2022

The trend of modelling native enzymes is increasing day by with the motive mechanistic elucidation enzyme-catalyzed reactions and efficiency catalysts. Most metalloenzymes are capable to activate molecular oxygen due presence metal ions. Among these, copper-based metalloenzyme catechol oxidase has an attractive feature its oxidizing ability generate ortho-quinones from catechol. Moreover, conve...

Journal: :The Journal of biological chemistry 1990
S N Gacheru P C Trackman M A Shah C Y O'Gara P Spacciapoli F T Greenaway H M Kagan

The spectral and catalytic properties of the copper cofactor in highly purified bovine aortic lysyl oxidase have been examined. As isolated, various preparations of purified lysyl oxidase are associated with 5-9 loosely bound copper atoms per molecule of enzyme which are removed by dialysis against EDTA. The enzyme also contains 0.99 +/- 0.10 g atom of tightly bound copper per 32-kDa monomer wh...

Journal: :The Journal of biological chemistry 2005
Agnes K Nagy David J Kane Chinh M Tran Robert A Farley Larry D Faller

Calcium pump-catalyzed (18)O exchange between inorganic phosphate and water was studied to test the hypothesis that all P-type pumps bind Mg(2+) before P(i) and validate utilization of the rate equation for ordered binding to interpret differences between site-directed mutants and wild-type enzyme. The results were remarkably similar to those obtained earlier with sodium pump (Kasho, V. N., Ste...

Journal: :Antimicrobial agents and chemotherapy 2001
J M Clements R P Beckett A Brown G Catlin M Lobell S Palan W Thomas M Whittaker S Wood S Salama P J Baker H F Rodgers V Barynin D W Rice M G Hunter

Peptide deformylase (PDF) is an essential bacterial metalloenzyme which deformylates the N-formylmethionine of newly synthesized polypeptides and as such represents a novel target for antibacterial chemotherapy. To identify novel PDF inhibitors, we screened a metalloenzyme inhibitor library and identified an N-formyl-hydroxylamine derivative, BB-3497, and a related natural hydroxamic acid antib...

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