conclusions multidrug resistance was significantly associated with mbl production in p. aeruginosa. early detection of mbl-producing p. aeruginosa and hospital antibiotic policy prescription helps proper antimicrobial therapy and avoidance of dissemination of these multidrug resistance isolates. background resistant pseudomonas aeruginosa is a serious concern for antimicrobial therapy, as the c...

1. Queenan AM, Bush K. Carbapenemases: the versatile β-lactamases. Clin Microbiol Rev. 2007;20:440–58. DOI: 10.1128/ CMR.00001-07 2. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: 20th informational supplement (update 2010 Jun). Document M100–S20-U. Wayne (PA): The Institute; 2010. 3. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, ...

The prevalence of infections caused by metallo-β-lactamase (MBL) expressing Gram-negative bacteria has grown at an alarming rate in recent years. Despite the fact that MBLs can deactivate virtually all β-lactam antibiotics, there are as of yet no approved drugs available that inhibit their activity. We here examine the ability of previously reported thiol-based MBL inhibitors to synergize with ...

Background: The opportunistic human pathogen, Pseudomonas aeruginosa, is a critical cause of nosocomial infection with high morbidity and mortality rate. Eradication P. aeruginosa has been troublesome due to its capacity develop strong multidrug resistance (MDR). Objectives: purposes this study were define the pattern antimicrobial sensitivity, typing, prevalence metallo-β-lactamase (MBL) detec...

Introduction: A large number of infectious processes are associated with opportunistic microorganisms. The phenotype antibiotic resistance such pathogens is multidrug-resistant strains the presence various β-lactamases. Our objective was to determine phenotypic and genotypic features staphylococci, enterobacteria, non-fermenting Gram-negative bacteria – cause diseases in patients health facilit...

BACKGROUND Carbapenem-resistant Acinetobacter baumannii (CRAb) is a major source of nosocomial infections in Spain associated with the production of OXA-58-like or OXA-24/40-like β-lactamase enzymes. We analysed the plasmids carrying the bla(OXA-24/40)-like gene in CRAb isolates obtained a decade apart. METHODS The presence of β-lactamases was screened for by PCR (metallo-β-lactamases, carbap...

Multidrug-resistant Enterobacteriaceae are emerging as a serious infectious disease challenge. These strains can accumulate many antibiotic resistance genes though horizontal transfer of genetic elements, those for β-lactamases being of particular concern. Some β-lactamases are active on a broad spectrum of β-lactams including the last-resort carbapenems. The gene for the broad-spectrum and car...