The last decade has witnessed dramatic advances in our ability to determine the levels of an ever broadening range of metabolites. That said, while transcriptomic approaches provide almost complete coverage (González-Ballester et al., 2010) and proteomics approaches are now capable of detecting upwards of 45% of the cellular protein complement (Baerenfaller et al., 2008), metabolomics is curren...

• A photosafety evaluation system of a chemical and its metabolites is required. Imipramine metabolite, desipramine, were employed as model compounds. Their was assessed based on the photochemical skin exposure data. The outcomes showed great contribution metabolite to phototoxicity. comprehensive evaluations would provide reliable information. Some chemicals have been reported cause metabolite...

Abstract Motivation Metabolite-protein interactions play an important role in regulating protein functions and metabolism. Yet, predictions of metabolite-protein using genome-scale metabolic networks are lacking. Here we fill this gap by presenting a computational framework, termed SARTRE, that employs features corresponding to shadow prices determined the context flux variability analysis pred...

Abstract Symbiosis of Penicillium rolfsii Y-1 is essential for the seed germination Hawaii yellow-eyed grass (Xyris complanata). However, local soil where plants grow naturally often suppresses radicle growth seedlings. This was drastically restored by coinoculation Paraburkholderia phenazinium isolate CK-PC1, which a rhizobacterium X. complanata. It found that CK-PC1 produced phenazine-1-carbo...

The post-genomic era is characterized by a range of high throughput profiling methods capable broadly characterizing gene expression levels, protein, and metabolite abundances. Application these methods, enzyme profiling, more recently, protein-metabolite interactions flux analysis have alongside modeling approaches allowed us to refine our understanding the regulation metabolism even in case c...

Microbial transformation of (+)-isomenthol (1) by various strains of fungi was investigated. Fusarium lini has successfully converted compound 1 into a new metabolite, 5α-hydroxyisomenthol (2), and a known metabolite, 1α-hydroxyisomenthol (3), whereas incubation with Rhizopus stolonifer only yielded metabolite 3. The transformed metabolites were structurally characterized on the basis of their ...

Despite recent technological advances, the analysis of biological samples for metabolite identification purposes often requires prior knowledge of the metabolite masses to successfully acquire high quality mass spectral data in the presence of intense background and interfering matrix signals. This, in turn, necessitates prior knowledge of the metabolite structure, which in most cases can be pr...

Phenacetin can be metabolized to reactive metabolites by a variety of mechanisms. (1) Phenacetin can be N-hydroxylated, and the resulting N-hydroxyphenacetin can be sulfated or glucuronidated. Whereas phenacetin N-O sulfate immediately rearranges to form a reactive metabolite which may covalently bind to protein, phenacetin N-O glucuronide slowly rearranges to form reactive metabolites. Incubat...

In the past decade, a global demand for products from organic agriculture has increased rapidly. Milk quality is of major interest for all parties. Therefore, the objective of this study was to compare cow performance and product quality in conventional and organic system. Twenty Holstein dairy cows were allotted to one of 2 diet groups, which including: a conventional diet (CON), and an organi...