The soybean apyrase, GS52, was previously characterized as an early nodulin that is expressed in roots and localized to the plasma membrane. Transgenic Lotus japonicus plants were constructed constitutively expressing the GS52 apyrase. Segregation and Southern-blot analysis identified four single-copy sense lines, several double-copy sense lines, and one double-copy antisense line for further a...

In bacteria, small non-coding RNAs (sRNAs) are critical regulators of cellular adaptation to changes in metabolism, physiology, or the external environment. In the last decade, more than 2000 of sRNA families have been reported in the Rfam database and have been shown to exert various regulatory functions in bacterial transcription and translation. However, little is known about sRNAs and their...

As an epiphyte orchid, Dendrobium catenatum relies on microorganisms for requisite nutrients. Metagenome pyrosequencing based on 16S rRNA and nifH genes was used to characterize the bacterial and diazotrophic communities associated with D. catenatum collected from 5 districts in China. Based on Meta-16S rRNA sequencing, 22 bacterial phyla and 699 genera were identified, distributed as 125 gener...

Rhizobium is a symbiotic biological nitrogen-fixing (BNF) bacteria. and has high diversity in different geographical regions worldwide. The BNF associated with the legume trees tropical environments improves efficiency of nitrogen increases soil organic matter, fertility. consists Mesorhizobium, Sinorhizobium, Bradyrhizobium, species belonging to each genera infecting specific host. This host s...

Samples of the root nodules were collected to isolate different species genus Rhizobium from several leguminous plants; Trigonella foenum-graecum, Medicago sativa, Lens culinaris, Vigna mungo, Vicia faba, Phaseolus vulgaris, and Cicer arietinum, based on their morphological, cultural, biochemical characteristics, in addition identification each at level by amplified polymerase chain reaction (P...