نتایج جستجو برای: mercaptoethanol
تعداد نتایج: 2124 فیلتر نتایج به سال:
* Present address, Department of Biochemistry, University of California, Berkeley 4, California. 1 The following abbreviations have been used; THF, tetrahydrofolic acid; lo-formyl-THF, N*o-formyltetrahydrofolic acid; 5,10-methenyl-THF, the cyclic N6-NrOimidazolinium derivative of 5-formyl-THF (anhydroleucovorin or anhydrocitrovorum factor) ; ATP, adenosine triphosphate; ADP, adenosine diphospha...
A substance which was mitogenic for murine B lymphocytes in the presence of 2-mercaptoethanol was isolated from agar. Stimulating activity of this material was stable to proteolysis or protein denaturants but was destroyed by periodate treatment. Agar-derived mitogen stimulation was distinct from that obtained with dextran sulfate or lipopolysaccharide and may define different populations of B ...
A metallamacrocycle containing twelve Zn2+ ions, making it the largest member of a family of pyrazole-bridged cyclic metal clusters, has been synthesized and structurally characterized from the reaction of [Zn(ClO4)2] with 5-methyl-3-phenylpyrazole, 2-mercaptoethanol, and NaOH.
A direct spectrophotometric method has been devised to follow the oxidation of tetrahydrofolate during the synthesis of thymidylate. It is based on the marked spectral change which occurs when 5, IO-methylenetetrahydrofolate is converted to dihydrofolate. A nonenzymatic reaction between formaldehyde and tetrahydrofolate yields 5,lO -methylenetetrahydrofolate (also called hydroxymethyltetrahydro...
Changes effected in purple uteroferrin’s resonance Raman spectrum by mercaptoethanol reduction suggest that the protein’s single iron atom is coordinated to more than one tyrosyl residue. Detection of a conservative pair of visible CD bands, indicative of exciton splitting of a tyrosinate-to-iron charge transfer band, strongly supports this suggestion. Additional features in the protein’s visib...
Modeccin was extracted from the root of Adenia digitata and purified by gel filtration on Sephadex G-100, ion exchange chromatography on DEAE-cellulose, and affinity chromatography on a column containing immobilized, neuraminidase-treated fetuin. The toxin was eluted from the affinity column with lactose. Polyacrylamide gel electrophoresis of affinity-purified modeccin in the presence of sodium...
The present study was designed to ascertain whether the negative effects on reproductive potential of post-ovulatory ageing in vitro of oocytes can be prevented by antioxidant therapy. Mouse metaphase II (MII) oocytes were aged in vitro for 12 h prior to insemination in the presence of varying concentrations of L-ascorbic acid, 6-methoxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), L...
BACKGROUND beta-III-neurotubulin is a characteristic degradation protein of cellular cytoskeleton of nervous tissues, which originates with disorders that lead to the loss of peripheral neurons or neurons of the central nervous system. For the diagnostics of neuropathic and neurodegenerative processes, beta-III-neurotubulin is therefore the goal structure, and radioactive labelled antibody TU-2...
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