Recently, we have shown that high molecular weight polymers inhibit cortical granule exocytosis at total osmolalities only slightly higher than that of sea water (Whitaker, M., and J. Zimmerberg. 1987. J. Physiol. 389:527-539). In this study, we visualize the step at which this inhibition occurs. Lytechinus pictus and Strongylocentrotus purpuratus eggs were exposed to 0.8 M stachyose or 40% (wt...

Accumulation of calcium in the mitochondria of rat liver parenchymal cells at 16 and 24 hours after poisoning with carbon tetrachloride is associated with an increase in amount of liver inorganic phosphate, the persistence of mitochondrial adenosine triphosphatase activity, and the formation of electron-opaque intramitochondrial masses in cells with increased calcium contents. These masses, whi...

The color of human eyes is often the same as iris eye, which itself consists two parts: anterior layer stroma and posterior or pigment epithelium. Based on experiments, it believed that determined based four factors: granules in epithelium, concentration stromal melanocytes, melanin pigments scattering absorption light from extracellular matrix properties stroma. Becomes. In general, melanocyte...

The iron storage macrophage has been Isolated from the marrow of Imforontreated mice and studied in vitro by morphologic, histochemical, and functional tests and isotope labeling techniques. Those macrophages on stained preparations are large, many times binucleate cells (up to 150 ii), and show Prussian blue reactivity. In Epon-embeddod, stained thick sections they contain elongated narrow bas...

Evidence obtained from both biochemical and morphological studies indicates that mitochondria play major roles in maintaining intraccllular ionic stability. In the case of whole cells, as well as for isolated mitochondria, an increase in size a n d / o r number of intramitochondrial granules is associated with the stabilizing process during changes in the ionic environment. The increase in larg...

neity simply that some proteins must be segregated to survive P inside the neutrophil, or for the neutrophil to survive, much OLYMORPHONUCLEAR leukocytes were discovered by Paul Ehrlich, when fixation and staining techniques as two-component glue has to be stored in two separate made it possible to identify the lobulated nucleus and the tubes, although both will be used at the same time? Whethe...

The main objective of the present study was to develop colon targeted drug delivery systems for Nitrofurantoin using guar gum as a carrier. Matrix tablets containing various proportions of guar gum were prepared by wet granulation technique using starch paste as a binder. The in-vitro drug release study was undertaken at 37±0.5°C in 0.1N Hcl for 2 hr; followed by pH 7.4 phosphate buffer (3hr) f...

Starch encapsulation has been proposed as a method for controlling the rate at which pesticides are released into the soil. Relatively ittle is known about what environmental f ctors influence controlled release. A series of laboratory studies were initiated to improve our understanding of how water potential, temperature, and soil microbial activity influence rate of release of starch-encapsul...

The proteins of trichocysts (secretory granules) from Paramecium tetraurelia have been biochemically characterized. Two-dimensional electrophoresis revealed 34 major components and at least 120 minor components, most with molecular weights ranging from 14,000 to 21,000 and isoelectric points ranging from 4.8 to 5.2. Comparison of two-dimensional electrophoretic patterns of trichocysts before an...

Immunolabelling of cultured cells for chondroitin-sulfate proteoglycan (CSPG), in conjunction with antibodies to fibronectin, collagen and laminin, revealed the spatial interrelationships between the different matrix components. CSPG was organized in two major forms. Fibronectin-independent dotted patterns of CSPG were detected on the substrate and cell surfaces at early stages after plating. A...