Mass spectrometry has brought unprecedented possibilities in the field of proteomics. The advances obtained in the last 10 years have been outstanding and have enabled faster and more reliable data acquisition and comparison. One powerful method developed was the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling (MALDI-TOF MS profiling) approach, primarily u...

Mycoplasma species identification is based on biochemical, immunological, and molecular methods that require several days for accurate identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a novel method for identification of bacteria and has recently been introduced into the clinical microbiology laboratory as a rapid and accurate techni...

Mastitis is one of the most economic disease affecting dairy cows worldwide. Its classic diagnosis using bacterial culture and biochemical findings is a difficult and prolonged method. In this research, using of matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF MS) permitted identification of different microorganisms with high accuracy and rapidity (only 24 ...

Fundamental concepts of high-resolution mass spectrometry as it relates to the issue of accurate mass measurements are presented. Important issues speciÐc to magnetic sector instruments, Fourier transform ion cyclotron resonance and time-of-Ñight (TOF) methods are discussed. Recent high-resolution TOF measurements on peptides and proteins, ionized by matrix-assisted laser desorption/ionization ...

List of papers This thesis is based on the papers listed below, which are referred to in the text by Roman numerals I-V. Neuropeptidomics: MS applied to the discovery of novel peptides from the brain. An automated method for scanning LC-MS data sets for significant peptides and proteins, including quantitative profiling and interactive confirmation. Striatal proteomic analysis suggests that fir...

Whole-cell fingerprinting by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) in combination with a dedicated bioinformatic software tool (MALDI Biotyper 2.0) was used to identify 152 staphylococcal strains corresponding to 22 staphylococcal species. Spectra of the 152 isolates, previously identified at the species level using a sodA gene-based oligonu...

Broberg, S. Studies of Oligoand Polysaccharides by MALDI-TOF and ESI-IT MS Mass Spectrometry. Doctor’s dissertation. ISSN 1401-6249, ISBN 91-576-6465-X This thesis presents methods for analyzing carbohydrates with matrix-assisted laser desorption/ionization time-of-flight, MALDI-TOF, and electrospray ionization ion trap multiple-stage, ESI-IT MS, mass spectrometry, MS. MALDI-TOF MS was employed...

Acinetobacter species are common inhabitants of freshwater and marine ecosystems with a capacity to induce disease in affected fish. To facilitate their rapid reliable identification, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was applied from The aims the study were, thus, identify profile tissues fish living vulnerable environment impacted by ...

We evaluated the performance of matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) associated with Bruker BioTyper TM V7.0.0 database for identification 713 bacterial strains isolated from seafood products and sea water samples (ANSES B3PA collection) under culture conditions that may have been significantly different those used to create reference spectrum...

Introducción: Ralstonia mannitolilytica es un agente causal de infecciones asociadas al cuidado la salud. Las especies del género son difíciles identificar y diferenciar. Nuestro objetivo fue tipificar aislamientos R. obtenidos hemocultivos pacientes pediátricos. Métodos: Colectamos 10 identificados con sistema automatizado, espectrometría masas analizador tiempo vuelo desorción mediante láser ...