Pseudomonas aeruginosa ExoU, a type III secretory toxin and major virulence factor with patatin-like phospholipase activity, is responsible for acute lung injury and sepsis in immunocompromised patients. Through use of a recently updated bacterial genome database, protein sequences predicted to be homologous to Ps. aeruginosa ExoU were identified in 17 other Pseudomonas species (Ps. fluorescens...

In this study, 24 standard nontuberculous mycobacteria (NTM) species strains including 12 slowly growing mycobacteria strains and 12 rapidly growing mycobacteria strains were subjected to drug susceptibility testing using microplate Alamar Blue assay-based 7H9 broth. The most active antimicrobial agents against the 24 NTM strains were streptomycin, amikacin, the fluoroquinolones, and the tetrac...

Two Trypanosoma vivax stocks were initiated in culture with tsetse or culture-derived metacyclics. They were propagated axenically as bloodstream trypomastigotes at 35 degrees C in 4% CO2 in air. Populations of trypanosomes were incubated with various concentrations of antitrypanosomal compounds, namely diminazene aceturate, quinapyramine sulphate, DL-alpha-difluoromethylornithine, isometamidiu...

High-throughput​ ​genetics​ ​is​ ​a ​ ​powerful ​ ​approach ​ ​to ​ ​identify​ ​new​ ​genes​ ​involved ​ ​in ​ ​bacterial carbon ​ ​catabolism.​ ​Here,​ ​we ​ ​used ​ ​genome-wide ​ ​fitness​ ​assays​ ​to ​ ​identify​ ​a ​ ​novel ​ ​pathway​ ​for 2-deoxy-D-ribose ​ ​catabolism​ ​in ​ ​​Pseudomonas​ ​simiae​ ​​WCS417.​ ​The ​ ​genes​ ​that​ ​were ​ ​important for​ ​deoxyribose ​ ​utilization ​ ​...

The phylogenetic interrelationships of 14 members of the genus Fusobacterium were investigated by performing a comparative analysis of the 16S rRNA sequences of these organisms. The sequence data revealed considerable intrageneric heterogeneity. The four species Fusobacterium nucleatum (including F. nucleatum subsp. nucleatum, F. nucleatum subsp. polymorphum, "F. nucleatum subsp. fusiforme," an...