A bifunctional and simple aptasensor was designed to one-spot simultaneously detect two analytes, lysozyme and ATP. The aptasensor was obtained by the electronic interaction between methyl violet (MV) and dsDNA. The dsDNA was obtained by hybridization of ATP aptamer and lysozyme aptamer. And we used the resonance light scattering (RLS) technique to detect the concentration of lysozyme and ATP. ...

Reduced lysozyme was renatured by sulfhydryl-disulfide interchange reactions at pH 8.0 in the presence of 4 M urea, with or without additives at 40 degrees C. In the absence of additives, the final folding yield of reduced lysozyme was approximately 40%. In the presence of sarcosine, glycerol, ammonium sulfate, N-acetyl glucosamine and glucose, its folding yields increased in all cases. In part...

In this study, the fermentation broth of recombinant Pichia pastoris strain ncy-2 was studied. After pretreatment, separation, and purification, lysozyme optimized using biofilm ion exchange separation. Finally, dry enzyme powder prepared by concentrating vacuum drying. The removal rate bacterial cells 99.99% when centrifuged at low temperature. optimum conditions were: transmembrane pressure 0...

Enterococcus faecalis is a commensal bacterium found in the gastrointestinal tract of most mammals, including humans, and is one of the leading causes of nosocomial infections. One of the hallmarks of E. faecalis pathogenesis is its unusual ability to tolerate high concentrations of lysozyme, which is an important innate immune component of the host. Previous studies have shown that the presenc...

Oral immunotherapy for food allergy has been the focus of a lot of attention recently. The patients have to eat allergenic food instead of eliminating it in this therapy and there is no established standard method yet. To promote clear understanding and improvement of oral immunotherapy, the present study using B10.A mice investigated the effect of multiple oral administration of a model antige...

approved by : The enzymatic activities of bacteriophage T4 lysozyme mutants with different structural stabilities were measured in the presence and absence of colloidal silica nanoparticles using a spectrophotometric technique and an agar plate bioassay. Spectrophotometric technique is based on the continuous reading of the change in turbidity of a bacterial suspension, while agar plate bioassa...

Soft-shelled turtle egg-white lysozyme was purified and sequenced. Lysozyme was reduced and carboxymethylated to fragment it with trypsin, V8 protease and CNBr. The peptides yielded were purified by RP-HPLC and sequenced. Every trypsin peptide was overlapped by V8 protease peptides and CNBr fragment. The amino acid sequence was compared with other lysozymes. This lysozyme has an extra Gly resid...

In order to increase the water content and the oxygen permeability of hydrogels used in themanufacture of contact lenses, the polar monomer Silicon Hydrogel Contact Lenses (SHCL), and 2-hydroxyethyl methacrylate (HEMA) were copolymerized with the hydrogels. Due to the presence ofpolar monomers in the conventional contact lenses, the major component of the human tear,lysozyme is extensively adso...

the cotton bollworm, helicoverpa armigera (hübner), which is known as one of the most important economic pests, can cause severe damage to different crops in iran and many countries of the world. the effect of five maize hybrids: sc260, sc500, sc700, sc704 and dc370 on lysozyme activity in the hemolymph of sixth instar of h. armigera using lytic zone assay and its association with some fitness ...