Mouse 3T3 cells and their Simian Virus 40-transformed derivatives (3T3SV) were used to assess the relationship of transformation, cell density, and growth control to the cellular distribution of newly synthesized glycosaminoglycan (GAG). Glucosamineand galactosamine-containing G A G were labeled equivalently by [all]glucose regardless of culture type, allowing incorporation into the various G A...

Rapid testing of electrocatalysts and corrosion resistant alloys accelerates discovery of promising new materials. Imaging amperometry, based on the deployment of colloidal particles as probes of the local current density, allows simultaneous electrochemical characterization of the entire composition space represented in a thin-film alloy "library" electrode. Previous work has shown that nanome...

The intracellular site of assembly of hepatic very low density lipoproteins has been investigated. Two endoplasmic reticulum fractions and Golgi vesicles (relatively free from endosomal contamination) were isolated from rat liver and the luminal contents were released. The apoB-containing entities were separated from the lumen of the endoplasmic reticulum and Golgi vesicles by an immunoaffinity...

Many types of colloidal particles possess a core-shell morphology. In this Article, we show that, if the core and shell densities differ, this morphology leads to an inherent density distribution for particles of finite polydispersity. If the shell is denser than the core, this density distribution implies an artificial narrowing of the particle size distribution as determined by disk centrifug...

We report accurate calculations of the particle density of states in the dipolar hard-sphere fluid. Implementing efficient and tailored Monte Carlo algorithms, we are able to explore, in equilibrium, the low temperature region where a phase separation between a dilute gas of chain ends and a high-density liquid of chain junctions has been predicted to occur. Our data clearly show that the densi...

Hepatitis C virus (HCV) infects 3% of the world population and is the leading cause of liver failure in the United States. A unique feature of HCV is that the viral particles are integral to very low-density lipoprotein (VLDL)-derived lipoprotein particles. The virus is assembled into VLDL in hepatocytes and released out of the cells together with VLDL. The virus then infects more hepatocytes b...