The assay of tritium-labelled substances in faeces requires conversion of tritium into either tritium gas (Payne and Done, 1958) or tritium-labelled water. Belcher (1961) and Kremenchuzky, Musso, Hoffbrand, and Viola (1967) used wet oxidation of faeces followed by liquid scintillation counting, while Burns and Glass (1963) used dry oxidation in a bomb calorimeter. There is a need for a reproduc...

Electrophoretic immunoblotting (EIB [Western blotting]) has emerged as the major method for verification of seropositivity for human immunodeficiency virus (HIV) and therefore needs to be thoroughly characterized. The specificities of three EIB systems, our own and two commercial systems, were studied with anticellular sera and serial dilutions of human sera. We demonstrated that in one system,...

Glycolipid synthetases can be assayed conveniently by incubating the lipid substrate with the radiosugar-labeled nucleotide in a small plastic scintillation vial. At the end of the incubation period, water and perchloric acid are added, then n-butanol, then a toluene-based scintillation cocktail. The radioactive lipid partitions into the scintillation fluid, leaving excess sugar nucleotide in t...