Plant lipid transfer proteins (LTPs) are members of the pathogenesis-related proteins (PR-14) and some of them exhibit activity against phytopathogenic fungi. To investigate whether rice LTP2 plays a role in antifungal activity, the coding region of an Iranian rice Ltp2 gene was cloned into expression vector pET24-d(+) and then expressed in Escherichia coli Rosetta strain (DE3). The potential a...

CMP-N-acetylneuraminate:lactosylceramide alpha-2,3-sialyltransferase is tightly associated with the luminal side of the Golgi membrane as is its lipid substrate, lactosylceramide. In order to understand the kinetics, properties, and regulation of this enzyme, it is necessary to alter the amount and type of substrate in the membrane while minimizing changes in the membrane environment or in the ...

Chair: Horacio Garda Session: Intracellular Lipid Trafficking Kentaro HANADA (11:30‐12:00) Phosphoregulation of lipid‐transfer proteins: a lesson from the ceramide transfer protein CERT. Antonella DE MATTEIS (12:00‐12:30) The phosphoinositides and the Golgi complex. Enrique POLITI (12:30‐12:50) Docosahexaenoic acid (DHA) and sphingosine‐1‐phosphate (S1P) promote survival and spatial reorganizat...

Yeast Sec14p acts as a phosphatidylinositol/phosphatidylcholine-transfer protein in vitro. In vivo, it is essential in promoting Golgi secretory function. Products of five genes named SFH1-SFH5 (Sec Fourteen Homologues 1-5) exhibit significant sequence homology to Sec14p and together they form the Sec14p family of lipid-transfer proteins. It is a diverse group of proteins with distinct subcellu...