We have developed a simple and rapid method for preparing DNA-binding protein extracts from mammalian cells. The protocol is derived from the large scale procedure of Dignam et al. (1) and utilizes hypotonic lysis followed by high salt extraction of nuclei. The technique described by Dignam has several drawbacks, including the need for large numbers of cells and lengthy incubation and dialysis ...

BACKGROUND Progress in the treatment of breathlessness at rest or on minimum exertion in patients with cancer requires a practical and valid method of measuring symptoms. A study was undertaken to explore the practicality, repeatability, and sensitivity of reading numbers as a form of exercise test in this group of patients. METHODS Thirty patients with cancer and 30 age matched healthy subje...

At present, among the problems faced by museums in Mexico figure need to increase visitor numbers, create new audiences, attract public and private funding, optimise their management models, establish competitive differences between themselves while adopting technologies. Thus, this study examines evaluation given visitors of four affiliated universities Puebla, Mexico, rating appeal. Hence, a ...

Museums often suffer from so‐called ‘hypercongestion’, wherein the number of visitors exceeds the capacity of the physical space of the museum. This can potentially be detrimental to the quality of visitors’ experiences, through disturbance by the behavior and presence of other visitors. Although this situation can be mitigated by managing visitors’ flow between spaces, a detailed analysis of v...

in this paper we introduce the root of a fuzzy number, and we present aniterative method to nd it, numerically. we present an algorithm to generatea sequence that can be converged to n-th root of a fuzzy number.

the sst decomposition method for solving system of linear equations make it possible to obtain the values of roots of the system with the specified accuracy as the limit of the sequence of some vectors. in this topic we are going to consider vectors as fuzzy vectors. we have considered a numerical example and tried to find out solution vector x in fuzzified form using method of sst decomposition.

Compared with other types of festival activities, the musical festivals can attract large crowds of visitors easily and quickly. When the value experience of visitors is high, their satisfaction and loyalty will also be higher. In activities, the high degree of satisfaction means higher loyalty. The hypothesis model in this study indicates the causal relationship between visitor value and degre...

Do children spontaneously represent spatial-numeric features of a task, even when it does not include printed numbers (Mix et al., 2016)? Sixty first grade students completed a novel spatial estimation task by seeking and finding pages in a 100-page book without printed page numbers. Children were shown pages 1 through 6 and 100, and then were asked, "Can you find page X?" Children's precision ...

one of the growth limiting factors of onion plants is root and basal plate rot disease, caused by fusarium oxysporum f. sp. cepae. in this research, eight iranian onion genotypes, azarshahr red, ghom white, herssin, isfahan dorcheh, kashan white, nishabour ishaghabad, yazd abarkoh, zanjan gholigheseh and two commercial genotypes, texas erly grano and yellow sweet spanish were investigated. geno...

Current literatures on urban parks and green spaces showed that people’s preference for visiting urban parks in various group users is different. People visit parks for various motives and activities, leading to several patterns of park usability. However, the patterns in urban park use in majority of Iran cities are not yet known. This study presented the preference for activity in urban...