OBJECTIVES To explore the luminescent oxygen channeling technology-based digoxin immunoassay (LOCI digoxin assay) for rapid detection of lily of the valley extract and convallatoxin. The potential in vitro binding of convallatoxin with Digibind was also evaluated. METHODS Aliquots of a drug-free serum pool and a digoxin serum pool were supplemented with lily of the valley extract or convallat...

The draft genome sequence of a nitrate- and phosphate-removing, Gram-positive Bacillus sp. with optimum growth at 37°C and pH 7 in nitrate broth (HiMedia M439) isolated from rhizosphere of a water lily, with a genome size of 5,465,157 bp and a G+C content of 35.0%, is reported here.

Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G(2) period of premeiosis and is present in mature pollen. PMCP and H1 were extracted from pollen mother cells with 5% perchloric acid and isolated by reverse-phase high-performance liquid chromatography. The amino acid compositio...

Five new spirostanol saponins and a new furostanol saponin were isolated from the fresh bulbs of Lilium candidum. Their structures were elucidated on the basis of spectroscopic analysis, including two-dimensional NMR spectroscopic techniques and the result of acid hydrolysis. The isolated saponins contained a branched triglycoside moiety assigned as O-alpha-L-rhamnopyranosyl-(1-->2)-O-[beta-D-g...

The mechanism of dormancy and dormancy release in lily (Lilium spp.) bulbs is still incomplete and ambiguous. Based on a summary of dormancy characteristics, we review the effects of low temperature on dormancy release. This paper highlights the metabolic changes in carbohydrates, endogenous hormones, free amino acids and phenols during low temperature storage, and the key stages in dormancy re...

Lilies (Localisation and InterLeaving stragIES) was developed to deal with a forest fire fighting planning environment. The apphcation required adaptive planning in a reactive and generative environment. To model the application, it was necessary for multiple agents to be developed which added the usual communication [15, 19] issues. The planning environment needed to deal with both reactive an...

Considering their economic importance, there has been relatively little research published on postharvest handling of cut lilies. Complicating this is the diversity of cultivar groups--asiatics, orientals, LA hybrids, OT hybrids, longiflorum and others. And, the diversity within each group is large and constantly growing as new cultivars are released. Cultivation practices and production locati...

The villin/gelsolin/fragmin superfamily is a major group of Ca2+-dependent actin-binding proteins (ABPs) involved in various cellular processes. Members of this superfamily typically possess three or six tandem gelsolin-like (G) domains, and each domain plays a distinct role in actin filament dynamics. Although the activities of most G domains have been characterized, the biochemical function o...

Experiments with D-glucose-6-P stereospecifically tritiated at C-6 showed that the myo-inositol-1 P synthase reaction catalyzed by both the enzyme from beef testis and from pollen of Lilium longiflorum proceeds with stereospecific loss of the pro-6R and incorporation of the pro-6S hydrogen into the product. The ring closure thus occurs in a retention mode at C-6 of the substrate, a finding at v...