Two or possibly three equivalents of N-bromoacetylacetazolamide interact with two histidine residues located in the active site region of human carbonic anhydrase B. The most rapid reaction occurs at the l-nitrogen of a histidine residue and this is followed by a slower alkylation of the 3-nitrogen of the same histidine and the 3-nitrogen of a second histidine. From a reaction mixture of N-brom...

Drug immunoassays with use of reagent strips have been introduced by the Ames Co. for theophylline and phenytoin assays. Their phenobarbital assay is based on the ais (Apoenzyme Reactivation Immunoassay System) principle. Thirty microlitres of diluted serum is applied to the reagent pad and the strip is read in the Seralyzer reflectance photometer after 85 s. Fifty-three serum samples assayed b...

We evaluated the performance of urinalysis dipsticks and microscopic urine sediment analysis as predictors of urinary tract infection (UTI) in patient visiting hospital. The samples were processed for macroscopic examination to observe Leukocyte esterase (LE) and Nitrite (NIT) by dipstick method, microscopic examination to observe pus cells, culture of urine sample on Blood agar and MacConkey a...

The performance of the leukocyte test area of the new multiple urine test strip, Multistix-10-SG, was evaluated in both field trials and model experiments. The detection limit was found to be adequate for routine purposes. Unspecific reactions are rare and generally indicated by the results of the other test areas.

Activated complement contributes significantly to reperfusion injury after ischemia. This study explores functional consequences of C1-esterase inhibitor (C1-INH) treatment after superior mesenteric artery occlusion (SMAO)/reperfusion using intravital microscopy. Thirty anesthetized, spontaneously breathing, male Sprague-Dawley rats underwent SMAO for 60 min followed by reperfusion (4 h). C1-es...