A series of new 2,4-difluorophenyl(piperidin-4-yl)methanone oxime derivatives 4a-h and 5a-e were synthesized by the reaction of 2,4-difluorophenyl(piperidin-4-yl)methanone oxime with various sulfonyl and acid chlorides. The synthesized compounds were characterized by elemental analyses, FTIR, 1 H NMR and LCMS spectral studies. All compounds were evaluated for in vitro antibacterial and antifung...

The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and ...

A series of twenty two novel 1-cyclopropyl-6-fluoro-4-oxo-7-(4-substituted piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid analogues have been synthesized, characterized ((1)H NMR, (13)C NMR and LCMS) and evaluated for their inhibitory activity on the proliferation of human caucasian acute lymphoblastic leukemia cells (CCRF-CEM), breast adenocarcinoma cells (MDA-MB-468) and human colon c...

A new indole alkaloid, naucline (1) together with four known alkaloids, angustine (2), angustidine (3), nauclefine (4) and naucletine (5), were isolated from the bark of Nauclea officinalis. The structures of all isolated compounds were elucidated with various spectroscopic methods such as 1D- and 2D- NMR, IR, UV and LCMS-IT-TOF. In addition to that of alkaloid 1, the complete 13C-NMR data of n...

In this technical note, a novel methodology is described which combines targeted and unknown screening in a single LCMS/MS analysis, using a QTRAP® hybrid tandem mass spectrometer / linear ion trap. In addition to providing screening for target and unknown analytes, this method also generates fullscan MS/MS spectra for the detected analytes, to be used for confirmation via searching against an ...

A new neolignan, 3,4-dimethoxy-3',4'-methylenedioxy-2,9-epoxy-6,7-cyclo-1,8-neolign-11-en-5(5H)-one, which has been named (+)-kunstlerone (1), together with six known alkaloids: (+)-norboldine (2), (+)-N-methylisococlaurine (3), (+)-cassythicine (4), (+)-laurotetanine (5), (+)-boldine (6) and (-)-pallidine (7), were isolated from the leaves of Beilschmiedia kunstleri. The structures were establ...

The aim of this pilot study was to evaluate the use of advanced proteomics techniques to identify novel protein markers that contribute to the transformation of benign meningiomas to more aggressive and malignant subtypes. Multiplex peptide stable isotope dimethyl labelling and nano-LCMS was used to identify and quantify the differentially expressed proteins in WHO Grade I, II and III meningiom...

Latent class models (LCM) represent the high dimensional data in a smaller dimensional space in terms of latent variables. They are able to automatically discover the patterns from the data. We present a topographic version of two LCMs for collaborative filtering and apply the models to a large collection of user ratings for films. Latent classes are topologically organized on a “star-like” str...

Celery (Apium graveolens L.) is a plant that belongs to the Apiaceae family and widely used as medical for low blood pressure, heart tonic, prevent cardiovascular disease. This study aims obtain flavonoid compounds, identify, test antioxidant activity of compounds crude extracts from celery leaves. The research procedures consisted four steps, first which was preliminary test. second step invol...