The carotenoids of yellow, red and tangerine tomato fruits were separated and identified by thin-layer chromatography (TLC). β-carotene is extracted with a mixture content hexan and acetone. The various systems of adsorbents and solvents for TLC of carote and tomato carotenes were examined, and it was revealed that the most suitable TLC system for separation and identification was silica gel pl...

The use of impregnated phases in thin-layer chromatography (TLC) has a long history, beginning in the very earliest days of the technique. As a consequence, much of the key literature in this Reld dates back to the 1960s and 1970s. The literature also contains a considerable number of examples of impregnation reagents where subsequently the methods have received little attention except in revie...

DURING A STUDY on the metabolism of histidine in a child affected with histidinemia (Beauvais, P., and Humbel, R., to be published), we became interested in the chromatography of imidazoles. In this disease the urine contains numerous imidazoles: histidine and its metabolites, imidazole-pyruvic, imidazole-lactic and imidazole-acetic acids and N-acetylhistidine.’I developed a chromatographic met...

Preparative HPLC 5}30 '20 2}20 0.01}1 High purity solvent required Higher resolution See also: II/Chromatography: Countercurrent Chromatography and High-Speed Countercurrent Chromatography: Instrumentation. Chromatography: Liquid: Countercurrent Liquid Chromatography. III/Alkaloids: Gas Chromatography; High-Speed Countercurrent Chromatography; Liquid Chromatography; Thin-Layer (Planar) Chromato...

A rapid semiquantitative method for the determination of anticonvulsant drugs in blood and urine by thin-layer chromatography is described that is applicable in most laboratories.Utilization of SilicAR TLC-7GF with benzene:acetone (4:1), chloroform:acetone(9:1), and carbontetrachloride:acetone(7:3) providesseparationof Dilantin,phenobarbital, Mysoline,Phenylethylmalondiamide, Mesantoin,and Nirv...

A systematic appraisal of a large number of solvent systems was carried out for the separation of lactic, pyruvic and 3-hydroxybutyric acids and all of the KREBS cycle acids, except oxalosuccinic, using silica gel G and cellulose thin layer Chromatographie plates. Four solvent systems which allowed separation of various combinations of acids are described and the limits of this method of separa...

Globalization forces analysts to demand extended control of variability in analytical measurements. A calculation procedure named the "error budget model" following recommendations proposed more than 20 years ago by the Bureau International des Poids et Mesures is established as a rule for evaluating and expressing the measurement uncertainty across a broad spectrum of measurements. This metrol...

NilEDICATIONS are one of the leading causes of fatal and nonfatal poisonings. Of all medications, salicylates (in children) and barbiturates (in adults) are most frequently involved in these poisonings. When these occur, the physician treating the victim may request a chemical analysis of a blood sample to confirm his clinical impression. A salicylate analysis is simple and rapid (1). However, ...

Bioautography is a microbial detection method hyphenated with planar chromatography techniques. It is based mainly on antimicrobial or antifungal properties of analyzed substances. The review discusses three versions of bioautography, i.e. contact, immersion and direct bioautography. The more concern is given to the last one. Many applications are quoted, not only for testing various groups of ...