AIMS To design and validate a colorimetric loop-mediated isothermal amplification assay for rapid detection of Phytophthora infestans DNA. METHODS AND RESULTS Two sets of loop-mediated isothermal amplification (LAMP) primers were designed and evaluated for their sensitivity and specificity for P. infestans. ITSII primers targeted a portion of the internal transcribed spacer region of ribosoma...

Image processing is an exciting concept in several digital applications for identifying features precisely. Hence, this technology chiefly utilized agriculture to predict the disease affection plat and leaves. However, complex image has reduced exactness rate of classification segmentation. In present work, potato leaves are normal, images considered. a novel Ant Lion-based Hyper-Parameter tune...

fire blight is an important worldwide disease of pome fruits that control of which through antibiotics is particularly critical. in this case and in absence of another chemical methods, the biocontrol methods can be considered as the best alternative for control of fire blight. in this study efficacy of 5 isolates of antagonistic bacteria belongs to pseudomonas fluorescens and pantoea agglomera...

Late blight, caused by phytophthora infestans, is a devastating disease in potato production. In severe cases, this can lead to crop failure. To rapidly detect late study, deep learning model was developed discriminate the degree of leaf diseases with high recognition accuracy and fast inference speed. It constructed total seven categories datasets single complex backgrounds, which were augment...

rots (Great Plains); seedling blight (Great Plains). Indian ricegrass {Oryzopsis hymenoides) : Seedling blight (far West) ; common root rot and crown rot (far West) ; rhizoctonia rot (scattered) ; snow molds (Pacific Northwest); seed rots (general); root necrosis and crown rots (far West). Kentucky bluegrass {Poa pratensis): Dollar spot (Northeast) ; brown patch (general); foot rot, Helminthosp...

Fire blight, caused by Erwinia amylovora (Ea), is a destructive disease of Malus (apple), Pyrus (pear) and some woody ornamentals in the rose family (Rosaceae). The goal of this project is to use a functional genomics approach to develop tools to breed fire blight resistant apples. Suppression subtractive hybridization (SSH) and cDNA-AFLP analysis were used to identify 650 expressed sequence ta...