Today, cryo-electron tomography (cryo-ET) is the only technique that can provide near-atomic resolution structural data on macromolecular complexes in situ. Owing to strong interaction of an electron with matter, high-resolution cryo-ET studies are limited specimens a thickness less than 200 nm, which restricts applicability peripheral regions cell. A complex workflow comprises preparation thin...

the need of aquarists for proper and safe disinfection of fish with chloramine-t (cl-t) necessitates toxico/pathologic examinations.   this study assays toxicity level of cl-t and histopathologic changes of gills, liver, kidney and brain of treated zebrafish (danio rerio). groups of 10 zebrafish were treated with 0 (control), 5, 10, 15, 30, 50,100 and 200 mg/l of cl– t. mortality was recorded a...

The microstructural evolution, deformation mechanism and mechanical properties of Fe-25Mn-3Si-3Al-0.3Nb steel in the process cold rolling were studied by optical microscopy, scanning transmission electron X-ray diffractometry, tensile testing microhardness tests. A high-density dislocation structure a small number strain-induced twins appeared microstructure at 30% strain. At 50% strain, austen...

Calcium-45 was injected into the dense albumen of fertile hen's eggs, to the extent of 25 microc. per egg. The eggs were incubated under standard conditions and three or more embryos removed daily and fixed in 10 per cent neutral formalin. Stripping-film autoradiograms were prepared from paraffin sections of the tibiofibulae. Exposure varied with the isotope concentration. The tissue sections w...

Pectic substances have generally been thought to be the principal binding agents between plant cells (12). These pectins are considered to be largely pectic acid because the middle lamella stains with ruthenium red (8). Ginzberg (7) considers that protein and metals are also important binding agents between the cells of the root tip of Alaska pea seedlings. There is, however, a need for further...

In this paper, we report a free-standing thin lamella consisting of nanoparticles with controllable shape. A self-assembly technique is utilized to obtain this sheet in a step by step fashion with nanoparticles and polymer single crystals as the basic building blocks. Inside the thin lamella, nanoparticles are not only immobilized on the surface of a polymer single crystal, which functions as a...

Three new species of the streptaxid snail genus Perrottetia are described from north and northeastern Thailand, Perrottetia aquilonaria sp. n., Perrottetia dermapyrrhosa sp. n. and Perrottetia phuphamanensis sp. n. Each species is endemic to a single or a few limestone mountain ranges. The species are characterized by the morphology of their genital organs, as well as by shell characters. Perro...

OBJECTIVE The objective of this study was to histologically clarify the difference of vascular wall damage when an ultrasonic scalpel is used in varied ways in the vicinity of a vessel. SUBJECTS AND METHODS 1) The surface of sodium carbonate-containing jelly was manually brushed with the edge of a dissecting hook type Harmonic Scalpel (HS), and the thickness of the air bubble layer was measur...

The impact of droplets at increased environmental pressure is important in many industrial applications. Previous studies mainly considered the process standard or reduced pressure, and effect high unclear. In this study, we experimentally investigate ethanol on dry smooth surfaces pressure. effects splashing rupture crown during are analyzed. results show that surrounding gas with can lead to ...