High-frequency oligonucleotide-directed recombination engineering (recombineering) has enabled rapid modification of several prokaryotic genomes to date. Here, we present a method for oligonucleotide-mediated recombineering in the model eukaryote and industrial production host Saccharomyces cerevisiae , which we call yeast oligo-mediated genome engineering (YOGE). Through a combination of overe...

BACKGROUND Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gene reporter constructs designed to investigate gene expression patterns in C. elegans. In our adaptation a rpsL-tet(A) positive/negative-selection cassett...

for a composition $lambda$ of $n$ our aim is to obtain reduced forms ‎for all the elements in the kazhdan-lusztig (right) cell containing ‎$w_{j(lambda)}$‎, ‎the longest element of the standard parabolic‎ ‎subgroup of $s_n$ corresponding to $lambda$‎. ‎we investigate how far this is possible to achieve by looking at‎ ‎elements of the form $w_{j(lambda)}d$‎, ‎where $d$ is a prefix of ‎an element...

Submit Manuscript | http://medcraveonline.com Abbreviations: LCT: Lambda-Cyhalothrin; LD: Lethal Dose; NOAEL: No-Observed-Adverse-Effect Level; Con A: Concanavalin A; SRBCs: Sheep Red Blood Cells; WBCs: White Blood Cells; Ig: Immunoglobulin; HA: Hemagglutination; QHS: Quantitative Hemolysis of SRBCs; FCS: Fetal Calf Serum; DTH: Delayed-type of Hypersensitivity Response; BMCs: Blood Mononuclear ...

Defects in the bacterial recB or recC or phage red or int gene did not impair ultraviolet light-induced clear mutations of lambda phage. Clear mutants of cI and cII genes were not enriched among RecA-promoted UV-induced recombinants between the N and O genes which closely encompass the c genes. No correlation was observed between UV-induced mutation and genetic recombination.

The aim of the present work is to introduce the concept of $lambda _{r}$-almost convergence of sequences. We define the spaces $fleft( lambda _{r}right) $ and $f_{0}left( lambda _{r}right) $ of $ lambda _{r}$-almost convergent and $lambda _{r}$-almost null sequences. We investigate some inclusion relations concerning those spaces with examples and we determine the $beta $- and $gamma $-duals of...