Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Leuconostoc, and Weissella in human feces. Analysis of fecal samples of four subjects revealed individual profiles of DNA fragments originating not only from species that ha...

A novel bacterium was isolated from a traditional fermented food, kimchi. The morphology, physiology, biochemical properties and 16S rRNA gene sequence of strain L133(T) were studied. Strain L133(T) was Gram-reaction-positive, catalase-negative and homofermentative, with rod-shaped cells that formed cream colonies. Cells grew in the presence of 0-5 % (w/v) NaCl (optimum, 1-2 %), at pH 5.0-9.0 (...

"Nostrano-cheeses" are traditional alpine cheeses made from raw cow's milk in Trentino-Alto Adige, Italy. This study identified lactic acid bacteria (LAB) developing during maturation of "Nostrano-cheeses" and evaluated their potential to produce γ-aminobutyric acid (GABA), an immunologically active compound and neurotransmitter. Cheese samples were collected on six cheese-making days, in three...

The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the pMP118 repA probe were also found in some strains of L. salivarius, showing for...

Professional antigen-presenting dendritic cells (DCs) are critical in regulating T cell immune responses at both systemic and mucosal sites. Many Lactobacillus species are normal members of the human gut microflora and most are regarded as safe when administered as probiotics. Because DCs can naturally or therapeutically encounter lactobacilli, we investigated the effects of several well define...

The taxonomic position of six Lactobacillus amylophilus strains isolated from swine waste-corn fermentations was reinvestigated. All strains were included in a multilocus sequence analysis (MLSA) study for species identification of Lactobacillus using the genes encoding the phenylalanyl-tRNA synthase alpha subunit (pheS) and RNA polymerase alpha subunit (rpoA). Partial pheS and rpoA gene sequen...

This work characterizes a set of lactobacilli strains isolated from the stomach of healthy humans that might serve as probiotic cultures. Ten different strains were recognized by rep-PCR and PFGE fingerprinting among 19 isolates from gastric biopsies and stomach juice samples. These strains belonged to five species, Lactobacillus gasseri (3), Lactobacillus reuteri (2), Lactobacillus vaginalis (...

Probiotics improve the intestinal microbiota and exert benefi ts for the host (Fuller, 1989; Parvez et al., 2006). Some strains of lactobacilli utilize lactose and alleviate lactose intolerance (Montalto et al., 2006). Lactobacillus gasseri is one of the predominant lactobacilli in the human intestine, and strains are used in probiotic foods. Lactobacillus gasseri is the major homofermentative ...

This study was carried out to examine aflatoxin B1 (AFB1) removal ability of four strains of lactic acidbacteria (LAB). Three indigenous (Lactobacillus rhamnosus TMU094, Lactobacillus fermentum TMU121and Pedioccus pentosaceus TMU457) and a non-indigenous (Labacillus rhamnosus PTCC1637) isolateswere studied. The strains were incubated with (AFB1) at different time. The toxin residual in the supe...

this study was carried out to examine aflatoxin b1 (afb1) removal ability of four strains of lactic acidbacteria (lab). three indigenous (lactobacillus rhamnosus tmu094, lactobacillus fermentum tmu121and pedioccus pentosaceus tmu457) and a non-indigenous (labacillus rhamnosus ptcc1637) isolateswere studied. the strains were incubated with (afb1) at different time. the toxin residual in the supe...