The metabolism of (14)C-labeled aspartic acid, diaminopimelic acid, malic acid and threonine by isolated pea (Pisum sativum L.) chloroplasts was examined. Light enhanced the incorporation of [(14)C] aspartic acid into soluble homoserine, isoleucine, lysine, methionine and threonine and protein-bound aspartic acid plus asparagine, isoleucine, lysine, and threonine. Lysine (2 millimolar) inhibite...

L-Asparagine is responsible for a major portion of the effect of peptone on the growth of L5178Y lymphoblasts in culture and is essential for the reproduction of these cells. Among the compounds tested, the requirement for the amide is replaced only by certain peptide derivatives of asparagine, and these are less active on a molar basis than is L-asparagine; L-aspartic acid, D-asparagine, and L...

In urea synthesis, according to the Krebs-Henseleit ornithine cycle (l), the transfer of nitrogen to citrulline to form arginine (Step II) was thought to occur from NH$. It was not until Cohen and Hayano (2) succeeded in demonstrating rapid arginine synthesis in liver homogenates from glutamic acid and citrulline that the transfer from an amino acid rather than from NH, was recognized as a main...

L-Asparagine is responsible for a major portion of the effect of peptone on the growth of L5178Y lymphoblasts in culture and is essential for the reproduction of these cells. Among the compounds tested, the requirement for the amide is replaced only by certain peptide derivatives of asparagine, and these are less active on a molar basis than is L-asparagine; L-aspartic acid, D-asparagine, and L...

Wright et al. (1949) announced the isolation in crystalline form of a naturally occurring complex of biotin from yeast, designated as biocytin. The possible metabolic role of biocytin in aspartic acid deaminase was studied by these investigators and the results showed that it was less active than an equivalent of biotin in the stimulation of this enzyme in aged bacterial suspensions. Inasmuch a...

Recently, Weiner and Hood (Science 190:987989, 1975) reported the use of an acid hydrolysis procedure in the study of the aspartic acid linkages of the soluble protein of the organic matrix of mollusk shells. Peptide bonds on both sides of aspartic acid residues have been shown to be particularly labile to hydrolysis by dilute acetic or hydrochloric acids (SCHULTZ et al, Biochemistry 1:694-698,...

L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) acrylamide reduction starch-rich foods cooked at temperatures above 100 °C, use a biosensor detection monitoring L-asparagine levels high relevance....

One of the tasks pharmaceutical science is to find new sources effective drugs. Such include plants such as Angelica archangelica L. and sylvestris L., which have been used for many years treat various diseases in folk medicine. Because chemical composition these poorly understood, aim our study was investigate amino acid leaves A. The acids species genus were determined by HPLC method. Eightee...

Aminophosphonic acids analogous to glutamic acid, aspartic acid, alanine, and valine were actively accumulated by Lactobacillus plantarum. Uptake was dependent on the availability of glucose and, in all cases, the estimated intracellular concentrations substantially exceeded extracellular levels. During uptake, there was little metabolism of tritiated 2-amino-3-phosphonopropionic acid (APP), th...

Polymerisation of α-amino acid N-carboxyanhydrides (NCAs) is one the most common techniques to prepare synthetic polypeptides. Of special interest are NCAs derived from acids, L-aspartic and L-glutamic acid, since investigations have been focused on their use synthesise multiblock copolypeptides or hybrid polypeptide-polymers design excipients suitable for delivery bioactive compounds. This per...