We report the first case of post-kala-azar dermal leishmaniasis due to Leishmania infantum in a human immunodeficiency virus type 1-infected patient in Australia. Molecular characterization of the isolate was performed using PCR restriction fragment length polymorphism targeting both repetitive sequences from Leishmania nuclear DNA and repetitive kinetoplast DNA minicircles for species differen...

The ancient question of trypanosome sexuality has recently been reactivated in view of important observations in the African species Trypanosoma brucie, in which Mendelian sexuality has been proposed as a working hypothesis on the basis o f indirect isozyme evidence. Subsequent experiments have confirmed that recombination can occur in T. brucei under defined experimental conditions and suggest...

In recent studies on kinetoplast DNA, the mitochondrial DNA of trypanosomatids (1), we had insufficient quantitites for standard electron microscopy (EM) procedures. We therefore developed a new microtechnique for sample preparation. EM of DNA is commonly done by the Kleinschmidt method (2), in which the nucleic acid is first adsorbed to a positively charged protein monolayer formed at an air-w...

Kinetoplast DNA (kDNA) is a novel form of mitochondrial DNA consisting of thousands of interlocked minicircles and 20-30 maxicircles. The minicircles replicate free of the kDNA network but nicks and gaps in the newly synthesized strands remain at the time of reattachment to the kDNA network. We show here that the steady-state population of replicated, network-associated minicircles only becomes...