α-Ketoglutaric acid (KGA) is a valuable compound with wide range of applications, e.g., in the cosmetics, pharmaceutical, chemical and food industries. The present study aimed to enhance efficiency KGA production by Yarrowia lipolytica CBS146773 from renewable carbon sources. In investigation, various factors that may potentially affect biosynthesis were examined bioreactor cultures performed o...

Carbonyl compounds are produced during fermentation and chemical oxidation during wine making and aging, and they are important to wine flavor and color stability. Since wine also contains these compounds as α-hydroxysulfonates as a result of their reaction with sulfur dioxide, an alkaline pre-treatment requiring oxygen exclusion has been used to release these bound carbonyls for analysis. By m...

CONTENTS Utilization of L-Glutamic and 2-Oxoglutaric Acid as Sole Sources of Carbon by EscheTichia coli BY Y. S. HALPERN and H. E. UMBAGER . . . . . . . . . . . The Cultural and Physiological Characters of the Pediococci BY H. L. G~~NTHER and H. R. WHITE Serological Characters of the Pediococci BY H. L. GUNTHER and H. R. WHITE . . . . The Production of Sporidesmin and Sporidesmolides by Pithomy...

Handler and Dann (1) suggested that nicotinamide is methylated in viva by methionine and excreted in the urine as N’-methylnicotinamide (NMeN). Perlzweig, Bernheim, and Bernheim (2) demonstrated that NMeN is formed in vitro by rat liver slices. The amounts formed were quite small and the maintenance of aerobic conditions and of cellular integrity was essential; furthermore, the addition of meth...

Ammonia is generally conceded to be the "key intermediate" in biological nitrogen fixation-that is, the compound which reacts to form the first stable organic compound, glutamic acid (Wilson and Burris, 1953). However, there is virtually no information concerning the reactions functioning in the conversion of N2 to ammonia. Reproducible nitrogen fixation by a cell-free preparation from bacteria...

In maize, a series of seed mutants with starchy endosperm could increase the lysine content by decreased amount of zeins, the main storage proteins in endosperm. Cloning and characterization of these mutants could reveal regulatory mechanisms for zeins accumulation in maize endosperm. Opaque7 (o7) is a classic maize starchy endosperm mutant with large effects on zeins accumulation and high lysi...

This work aimed to determine the formation over time of 3-methylbutanal and 3-methylbutan-1-ol recognized as malty during manufacture Raclette-type cheese fermention reconstituted skim milk, filter-sterilized MRS broth. Using dynamic headspace-vacuum transfer in trap extraction followed by gas chromatography coupled with mass spectrometry–olfactometry (DHS-VTT-GC-MS-O) a screening method for co...

Kynurenine aminotransferase from Pyrococcus horikoshii OT3 (PhKAT), which is a homodimeric protein, catalyzes the conversion of kynurenine (KYN) to kynurenic acid (KYNA). We analyzed the transaminase reaction mechanisms of this protein with pyridoxal-5'-phosphate (PLP), KYN and α-ketoglutaric acid (2OG) or oxaloacetic acid (OXA). 2OG significantly inhibited KAT activities in kinetic analyses, s...