BACKGROUND A century after its discovery, Chagas disease still represents a major neglected tropical threat. Accurate diagnostics tools as well as surrogate markers of parasitological response to treatment are research priorities in the field. The purpose of this study was to evaluate the performance of PCR methods in detection of Trypanosoma cruzi DNA by an external quality evaluation. METHO...

We studied the intramitochondrial localization of several multiprotein complexes involved in U-insertion/deletion RNA editing in trypanosome mitochondria. The editing complexes are located in one or two antipodal nodes adjacent to the kinetoplast DNA (kDNA) disk, which are distinct from but associated with the minicircle catenation nodes. In some cases the proteins are in a bilateral sheet conf...

abstract        background and objective: chabahar is in southern iran located near the iran-pakistan border. since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of leishmania parasite in the patients referred to the central laboratory.       methods: this descriptive cross-sectional study was conducted in 2011-2012 ...

visceral leishmaniasis (vl) is an emerging zoonosis disease in countries of the mediterranean basin caused by leishmania infantum. although domestic dogs are the main vertebrate hosts, many wild carnivores have been considered playing a role in the spreading of vl. sporadic numbers of dog and human vl have been reported in golestan province in north iran. the present study was performed to dete...

Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme e...

visceral leishmaniasis (kala-azar) is a serious health problem in some northern and south western parts of iran. the incidence of kala-azar caused by leishmania infantum has recently increased in nourabad-mamassani district of fars province, in the south of the country. this study was designed to determine the role of asymptomatic dogs as host reservoir of l. infantum in this new formed focus a...