In a search for microorganisms antagonistic to bacteria, especially to the gram-negative ones, the bacterial-agar plate method was used (Waksman and Woodruff, 1940a). This consists in adding a suspension of washed living bacteria to 1.5 per cent sterile washed agar containing 0.5 per cent NaCl and 0.5 per cent K2HPO4. To a fresh field or garden soil, or one previously enriched several times wit...

OBJECTIVE Evaluation of an analytic method for determining linezolid concentrations in biological fluids including plasma, vitreous humour and cerebrospinal fluid using high-efficiency liquid chromatography and subsequent ultraviolet detection. METHOD The method was validated by studying the following parameters: accuracy, precision, sensitivity, linearity and recovery. The drug was extracted...

A coupled system for arsenic speciation determination based on high-performance liquid chromatography (HPLC), on-line UV photooxidation and continuous-flow hydride generation atomic absorption spectrometry (HGAAS) was built from commercially available modules with minor modifications to the electronic interface, the software and the gas-liquid separator. The best results were obtained with stro...

Several studies have reported on the capabilities of different yeasts to utilize hydrocarbons hence the need to ascertain the petroleum hydrocarbon biodegradation potential of yeast isolated from palm wine. The study aimed at isolation, characterization and testing of yeasts from palm wine for biodegradation of petroleum hydrocarbons. Hydrocarbon utilizing yeasts (HUY) were isolated from palm w...

The O-acetylation of sialic acids turns out to be one of the most important modifications that influence the diverse biological and pathophysiological properties of glycoconjugates in animals and microorganisms. To understand the functions of this esterification, knowledge of the properties, structures and regulation of expression of the enzymes involved is essential. Attempts to solubilise, pu...

The chitinase producing strain TKU008 was isolated from the soil in Taiwan, and it was identified as a new species of Pseudomonas. The culture condition suitable for production of chitinase was found to be shaken at 30 degrees C for 4 days in 100 mL of medium containing 1% shrimp and crab shell powder, 0.1 % K2HPO4 and 0.05% MgSO4 . 7 H2O (pH 7). The TKU008 chitinase was suppressed by the simul...

Three halotolerant bacterial strains; Rhodobacter sphaeroides ES16 (the wild type) and the two mutant strains of R. sphaeroides ES16, namely N20 and U7, were cultivated in glutamate-malate (GM) medium and screened for production of polyhydroxybutyrate (PHB). The mutant strains N20 and U7 were found to accumulate PHB (53.9 and 42.0% of DCW, respectively) 3.6 and 2.8 times higher than the wild ty...

A high-performance liquid chromatographic method with amperometric detection has been developed for the determination of the diuretic bumetanide using a microBondapak C18 column. The mobile phase consists of a 50:50 acetonitrile-water mixture containing 5mM KH2PO4-K2HPO4 (pH 4.0). The compound is monitored at +1350 mV with an amperometric detector equipped with a glassy carbon working electrode...

Streptomyces strains isolated from Nelliyampathy forest soil of Western Ghats, Kerala, India were evaluated for their antibacterial efficacy against two indicator pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Among 140 strains tested, sixteen recorded potent antibacterial properties and were further screened against eleven bacterial pathogens. A strain identified as Streptom...

A moderately halotolerant Streptomyces strain, designated JAJ13 was characterized and a culture medium was statistically optimized to improve its antibacterial activity. Based on the phenotypic and molecular characteristics, strain JAJ13 was identified as a moderately halotolerant Streptomyces sp. JAJ13. Novelty of the strain JAJ13 in production of antibacterial compound was assessed by sequenc...