As acute nonlymphocytic leukemia (ANLL) with inv(16) (p13q22) or t(16;16)(p13;q22) has been shown to result from the fusion of transcription factor subunit core binding factor (CBFB) to a myosin heavy chain (MYH11), we sought to design methods to detect this rearrangement using reverse transcriptase-polymerase chain reaction (RT-PCR). In all of 27 inv(16)(p13q22) and four t(16;16)(p13;q22) case...

The only family 1 glycoside hydrolase in Clostridium cellulolyticum H10 (CcGH1) is annotated as a beta-galactosidase but has high sequence homology with many beta-glucosidases. Given the possible importance of beta-glucosidase in cellulose utilization by C. cellulolyticum, the encoding open reading frame Ccel_0374 was cloned and expressed in E. coli as a soluble fusion protein with thioredoxin....

We adapted a fusion polymerase chain reaction (PCR) strategy to synthesize gene disruption alleles of any sequenced yeast gene of interest. The first step of the construction is to amplify sequences flanking the reading frame we want to disrupt and to amplify the selectable marker sequence. Then we fuse the upstream fragment to the marker sequence by fusion PCR, isolate this product and fuse it...

In real applications, how to measure the uncertain degree of sensor reports before applying sensor data fusion is a big challenge. In this paper, in the frame of Dempster-Shafer evidence theory, a weighted belief entropy based on Deng entropy is proposed to quantify the uncertainty of uncertain information. The weight of the proposed belief entropy is based on the relative scale of a propositio...

A holey perfect Mendelsohn design of type h n 1 1 h n 2 2 h n k k (HPMD(h n 1 1 h n 2 2 h n k k) with block size four is equivalent to a frame idempotent quasigroup satisfying Stein's third law with the same type, where a frame quasigroup of type h n 1

To identify Rns-regulated genes, a maltose binding protein (MBP)-Rns fusion protein was used to isolate DNA fragments from enterotoxigenic Escherichia coli genomic DNA that carry Rns binding sites. In vivo transcription fusion analysis shows that Rns positively regulates the expression of the open reading frame of yiiS, which lies immediately downstream of one MBP-Rns-bound fragment.

Let H be a Hilbert space. Given a bounded positive definite operator S on H, and a bounded sequence c = {ck}k∈N of non negative real numbers, the pair (S, c) is frame admissible, if there exists a frame {fk}k∈N on H with frame operator S, such that ‖fk‖ 2 = ck, k ∈ N. We relate the existence of such frames with the Schur-Horn theorem of majorization, and give a reformulation of the extended ver...

The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (Deltahag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases.

$K$-frames as a generalization of frames were introduced by L. Gu{a}vruc{t}a to study atomic systems on Hilbert spaces which allows, in a stable way, to reconstruct elements from the range of the bounded linear operator $K$ in a Hilbert space. Recently some generalizations of this concept are introduced and some of its difference with ordinary frames are studied. In this paper, we give a new ge...

Text summarization is a method of retrieving information, and it very effective. approach techniques fall into two categories: abstract abstract. This white paper focuses on extraction so that the output application key sentence taken verbatim from original text without verbal modification. There are four main processes in this research. These preprocessing phase, scoring function, optimization...