نتایج جستجو برای: isotope detection
تعداد نتایج: 597360 فیلتر نتایج به سال:
The fovea is a declivity of the retinal surface associated with maximum visual acuity. Foveae are widespread across vertebrates, but among mammals they are restricted to haplorhine primates (tarsiers, monkeys, apes, and humans), which are primarily diurnal. Thus primates have long contributed to the view that foveae are functional adaptations to diurnality. The foveae of tarsiers, which are noc...
We report a cost efficient approach for amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. The method provides an economically advantageous alternative to recently established protocol for isotopic labeling using expensive synthetic media. The method is based on cultivation of the L. tarentolae expression strain in a cheap complex medium supplemented with...
We have developed a transportable spectroscopic nitrogen isotopic analyzer. The spectrometer is based on dual-modulation Faraday rotation spectroscopy of nitric oxide isotopologues with near shot-noise limited performance and baseline-free operation. Noise analysis indicates minor isotope ((15)NO) detection sensitivity of 0.36 ppbv·Hz(-1/2), corresponding to noise-equivalent Faraday rotation an...
In order to monitor the behaviour of contaminants in the aqueous environment effective enrichment techniques often have to be employed due to their low concentrations. In this work a robust and sensitive purge and trap-gas chromatography-isotope ratio mass spectrometry method for carbon and hydrogen isotope analysis of fuel oxygenates in water is presented. The method evaluation included the de...
A gas chromatography electron capture negative ionization mass spectrometry (GC(ECNI)MS) procedure for the determination of priority polybrominated diphenyl ethers (PBDEs; congeners 28, 47, 99, 100, 153 and 154) in water samples at regulatory EU levels has been developed. The method is based on the use of (81)Br-labelled PBDEs for isotope dilution analysis and the measurement of (79)Br/(81)Br i...
A method for the determination of creatinine in human serum by isotope dilution-mass spectrometry is described. The analytical procedure comprises the following steps: Addition of [13C,15N2 )creatinine to the serum sample; ion exchange chromatography on the cation exchange resin AG 50W-X2; formation of the trimethylsilyl derivative; gas liquid chromatography-mass spectrometry (GC-MS); selected ...
Chloroform has been for a long time considered only as an anthropogenic contaminant. The presence of chloroform in forest soil and groundwater has been widely demonstrated. The frequent detection of chloroform in groundwater in absence of other contaminants suggests that chloroform is likely produced naturally. Compound-specific isotope analysis of chloroform was performed on soil-gas and groun...
OBJECTIVE The assay of DNA mismatch repair (MMR) activity can be used as a biomarker for environmental condition detection and human disease diagnosis. Radioactive ³²P-endlabeled DNA containing mismatch is extensively used as the substrate for MMR activity analyses. The aim of the present study is to develop a simple non-radioactive, but equally specific and sensitive method for the MMR activit...
The Gas Chromatograph Mass Spectrometer (GCMS) on the Huygens Probe will measure the chemical composition of Titan’s atmosphere from 170 km altitude (∼1 hPa) to the surface (∼1500 hPa) and determine the isotope ratios of the major gaseous constituents. The GCMS will also analyze gas samples from the Aerosol Collector Pyrolyser (ACP) and may be able to investigate the composition (including isot...
The only naturally occurring isotope of bismuth, 209Bi, is commonly regarded as the heaviest stable isotope. But like most other heavy nuclei abundant in nature and characterized by an exceptionally long lifetime, it is metastable with respect to alpha-decay. However, the decay usually evades observation because the nuclear structure of 209Bi gives rise to an extremely low decay probability and...
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