1. The movement of normal and sensitized red blood cells in the electric field is a function of the hydrogen ion concentration. The isoelectric point, at which no movement occurs, corresponds with pH 4.6. 2. On the alkaline side of the isoelectric point the charge carried is negative and increases with the alkalinity. On the acid side the charge is positive and increases with the acidity. 3. On...

According tó the literature there is ho doubt about the structure of nicotinic acid in mineral acid or alkali hydroxide solutions. However, rather controversial opinions on the structure of the substance can be found in the literature when the state at the isoelectric point is considered. Structure J [1], II [2—4] or an equilibrium mixture [5] have been assigned to nicotinic acid at the isoelec...

It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a ge...

Two glycoproteins were purified and biochemically characterized from the lichen X. parietina. Both behaved as enzymes with arginase activity and haemaglutinins. Secreted arginase (SA) contained galactose and glucose in the saccharide moiety and an isoelectric point of 4.54. The algal binding-protein (ABP) had N-acetyl-glucosamine and glucose as glycosidic residues and an isoelectric point of 3....

This paper deals with some basic proteins of cell nuclei which differ in a number of ways from the classical histones of Kossel. Two characteristics of a histone noted by Kossel are its high arginine content and its way of being precipitated on addition of an excess of ammonium hydroxide (1-2). A high arginine content would tend to give a protein a basic isoelectric point and precipitability in...

Previous studies have shown that in mammalian cells proteins of large molecular weight are degraded more rapidly than small ones. Evidence is presented here that half-lives of proteins are also related to their isoelectric points. A double-isotope method was used to compare degradative rates of soluble proteins separated by isoelectric focusing. In rat liver, skeletal muscle, kidney, and brain,...

An algorithm to estimate the transient ST segment level and to construct the ST segment-level functions from 24-hour ambulatory ECG records is presented. The algorithm was developed and tested using the Long-Term ST Database (LTST DB). Initially, the average heart beats are constructed from normal and non-noisy heart beats of the records of the database in a 16-second neighborhood of each heart...