Capillary electrochromatography (CEC) combines the separation principle of HPLC (partitioning between mobile and stationary phases) with the high efficiency of capillary electroseparation methods. In CEC the electroosmotic flow (EOF) inherent in capillary electrophoretic separations is used to transport solute and mobile phase through a packed capillary column. The properties of the EOF provide...

For the diagnosis of cystic fibrosis, we use the "Macroduct Sweat Collection System" (Wescor) to sample sweat, and anion-exchange chromatography to determine its chloride content. The chromatographic system consists of a Whatman Partisil SAX 10-microns column, an isocratic phosphate-buffered mobile phase, and ultraviolet detection at 200 nm. The sweat chloride concentrations of 10 individuals w...

A simple, selective, rapid and precise reverse phase HPLC method has been developed for the simultaneous estimation of Ofloxacin and Satranidazole in tablets. The analyte was resolved by using a mobile phase 0.05 M phosphate buffer and acetonitrile in the ratio of 65:35 v/v at a flow rate of 1.0 ml/min on an isocratic HPLC system at a wavelength of 320 nm. The linearity was obtained in the conc...

We investigated the use of a diode array detector in conjunction with isocratic cation-exchange liquid chromatography for detection of organic acids in urine. The spectra and retention times of abnormal peaks found on chromatography of urine from patients with methylmalonic aciduria, maple syrup urine disease, and lactic aciduria were recorded and compared with those obtained for group of pure ...

Two high-performance liquid chromatographic methods are compared for the determination of monoand oligosaccharides in vegetables using an N H 2 column and acetonitrile-water as the mobile phase. One method uses a gradient elution and evaporative light-scattering detection; method precision (relative standard deviation) ranges from 1.09 to 4.44%, and detection limits range from 0.014 to 0.061 mg...

A reversed-phase high-performance liquid chromatographic method has been developed to quantitatively determine matrine in liquid crystal nanoparticles. The chromatographic method is carried out using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min with SPD-20A UV/vis detector and the detection wavelength was a...

The Agilent 1100 Series preparative pump1,2 forms the basis of the Agilent 1100 Series purification system. It is an isocratic high performance pump with dual piston design for a flow rate range of 5–100 ml/min at 400 bar. To form a gradient system two pumps have to be combined. The extremely small delay volume (figure 1) of the mixer and the attached capillaries, about 0.7 ml, allows fast and ...

A high performance liquid chromatographic method with UV detection was developed and validated for simultaneous determination of Pioglitazone and Clopidogrel. Separation was performed on a C18 column by isocratic elution with a mobile phase of Methanol: Acetonitrile : Water (80:10:10) at pH 4.6. The UV detection was set at 230 nm. The method proved to be specific, accurate, precise and linear o...

A rapid, reversed-phase "high-performance" liquid-chromatographic separation of the five naturally occurring cyclic ribonucleotides is described. The separation, optimized for the measurement of these compounds in biological samples, is short (25 min), sensitive (50-100 pmol), and requires no sample pre-concentration steps. We also report an alternative isocratic elution mode, optimized for a r...

This application note describes the use of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation procedure. The QuEChERS method employs an extraction and cleanup protocol for the determination of acrylamide with C3-acrylamide as the internal standard. The analyte and internal standard were separated on a reversed phase C-18 column (2.1 mm × 150 mm, 3 μm) by isocratic e...