The objective of the current study was to evaluate the reproducibility of a reverse transcriptase PCR (RT-PCR)-based technique to differentiate viable and dead Salmonella cells in raw and sterilized milk. The microorganism was initially inoculated into the milk samples followed by incubating at 37°C for 4 h prior to inactivation by heat at 80°C for 10 min. The treated and non-treated samples we...

Se realizó una revisión sistemática de los métodos diagnóstico descritos en la literatura para identificación y caracterización especies del género Salmonella. La se utilizando publicaciones 2015 a 2021 registradas las bases datos NCBI/PubMed, ScienceDirect, Scopus, SciELO, Google Scholar Springer, aplicando metodología PRISMA. analizaron 61 artículos, agrupando según el país origen, tipo muest...

A total of 150 fecal and water samples from four swine farms were tested for the presence of Salmonella enterica using different enrichment techniques as follows: (i) 92 fecal samples from nursery and farrowing barns at three swine farms were preenriched overnight in tryptic soy broth (TSB) at 37 degrees C followed by overnight enrichment in Rappaport-Vassiliadis 10 broth (RV10) at 42 degrees C...

I read with great interest an article of Ez-zatzadegan, et al, recently published in IJOTM [1]. They reported their experience about the incidence and etiology of inva-sive fungal infections (IFIs) among 471 kidney transplant recipients within a decade. I agree that the recent reports show a decline in the number of IFIs after kidney transplan-tation; however, IFIs are associated with high mort...

  A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad. The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping. PCR amplification of invA gene as a specific method for detection of Salmonella w...

Despite a long research history, the lactate threshold continues to be widely controversial field. Notwithstanding controversies and influence of multiple determinants, estimation in sports has been considered one essential parameters prescription training intensity, load monitoring, perfor- mance prediction. The study aimed compare anaerobic ventilatory thresholds as determined by different me...

ABSTRACT Salmonella is a foodborne pathogen that has contributed to numerous food safety accidents worldwide, making it necessary detect contamination at an early stage. A pair of specific primers based on the invA gene was designed for PCR. Target double-stranded DNA (dsDNA) from PCR purified and denatured high temperature obtain target single-stranded (ssDNA). Two carbo...