In most species examined to date, spermatozoa mature during transit through the epididymis to become functionally competent to swim progressively, fertilize oocytes, and produce viable offspring. In the studies presented here, the status of spermatozoa obtained from the regions of the epididymis of the cynomolgus monkey (Macaca fascicularis) was examined. Epididymal fluid was collected by micro...

Background: Excess reactive oxygen species (ROS) in the semen is believed to affect fertility in men. Morphologically abnormal sperms and their relation to seminal oxidative stress in infertile and subfertile men are not clear. Objective: To correlate various sperm morphological defects with seminal oxidative stress in infertile and subfertile men. Materials and Methods: The study included 25 p...

We investigated the identity and quantitative variations of proteins extracted from human sperm heads using a label-free Gel-MS approach. Sperm samples were obtained from three men with high sperm counts at three different time points. This design allowed us to analyse intra-individual and inter-individual variations of the human sperm head proteome. Each time point was analyzed in triplicate t...

Recent studies have shown cytoplasmic droplets to be normal morphological occurrences in human male spermatozoa. When the cytoplasm around the sperm midpiece is present in large amounts, however, pathological effects may transpire. The cytoplasmic droplet then becomes known as excess residual cytoplasm, which can impair overall sperm function and produce higher levels of reactive oxygen species...

Background: Diabetes mellitus (DM), primary or idiopathic, is a chronic disorder of the carbohydrate, lipid and protein metabolism, characterized through hyperglycemia and glycosuria. DM can involve male reproductive function at many levels. Vitamin C ( ascorbic acid) is one of necessary elements in human and mammalian diet that is very important in fertility. Vitamin C prevent from gamets dama...

Cytological and autoradiographic studies were done to investigate the cytoplasmic control of DNA synthesis under conditions of physiological polyspermy. The DNA synthetic phases of the egg, principal sperm and accessory sperm nucleic were determined and correlated with nuclear morphology and develpmental fate. Results show that accessory sperm nuclei undergo morphological transition to pronucle...

The relationship between cytoplasmic incompatibility and the obligate intracellular alphaproteobacteria Wolbachia has for a long time been reported. Although molecular mechanisms responsible this reproductive alteration are beginning to be understood, effects of on germ cell structure dynamics have not yet fully investigated. We report here that presence in infected cysts elongating spermatids ...

Abstract Study question Will use of surgically retrieved Testicular Sperms (TESA) in Intra-Cytoplasmic Sperm Injection (ICSI) cycles increase embryo aneuploidy? Summary answer Use TESA sperms for ICSI seems safe and doesn’t aneuploidy. performed various indications also showed no variation with What is known already Invention improved surgical sperm retrieval techniques have helped men severe m...

We present live births resulting from two separate IVF cycles in a couple in which ICSI was performed with sperm specifically selected for presence of small cytoplasmic droplets. These cycles followed previous cycles using standard sperm selection methods in which very poor embryo development and no pregnancies ensued. The male partner was diagnosed with severe male factor infertility including...

Objective: Our study was undertaken to find if any correlation exists between levels of Anti-Mullerian (AMH), follicle stimulating hormone (FSH) and estradiol (E2), with the ovarian reserve. Methods: A Randomized, prospective study was conducted at Infertility Clinic,Department of Obstetrics & Gynecology, Mahatma Gandhi Medical College and Hospital,Jaipur on 105 patients aged 25-40 years underg...