OBJECTIVE To examine the effect of sperm morphology on embryo development at the chromosomal level. DESIGN Prospective study. SETTING Assisted fertilization center. PATIENT(S) Couples who underwent IVF-PGS cycle, as a result of advanced maternal age, were randomly allocated into two groups: intracytoplasmic sperm injection (ICSI; n = 60) or intracytoplasmic morphologically selected sperm ...

Intracytoplasmic sperm injection (ICSI) is a delicate procedure requiring considerable skills of the person performing it. Theoretically, the injection procedure could damage cytoplasmic structures in the oocyte, resulting in sublethal cellular injury and/or numerical chromosomal abnormalities that could lead to impaired embryonic development. In the present study, features of the injection pro...

objective: due to the worldwide increase in infertility, it is both necessary and important to have assisted reproductive technology (art) registries. in iran, donation and surrogacy programs are approved by decrees from religious scholars. art has been used since 1984 in iran and the first iranian infant conceived by gamete intra-fallopian transfer (gift) was born in 1989. this report, however...

The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...

background: implantation failure is the most important cause of recurrent in vitro fertilization/intra cytoplasmic sperm injection (ivf/icsi) failure. several reports suggest that intercourse during the peritransfer period might improve pregnancy rates. this study is designed to determine whether intercourse during the peritransfer period will improve pregnancy and implantation rates in patient...

background: auto antibodies to zona-pellucida (aza) seem to be important autoantibodies implicated in reproduction, with substantial role in both endocrine and reproductive functions of the human ovary. there are some debates on the relation of aza with infertility, repeated in vitro fertilization (ivf) attempts, and outcome of it. objective: in this study, we assessed the presence of aza in th...

Background: Intracytoplasmic Sperm Injection (ICSI) is one of the methods of assisted reproductive technology that aims to successfully treat male factor infertility in humans and domestic animals. The technique involves mechanically injecting a single sperm or sperm nucleus with the help of a micromanipulator into the cytoplasm of a mature metaphase II oocyte. Results: In domestic animals, ICS...

BACKGROUND Cryptozoospermia (CO) is a situation in which spermatozoa cannot be observed in a fresh semen sample unless an extended centrifugation and microscopic search are performed. CO patients are suggested to use only intracytoplasmic sperm injection (ICSI) as infertility treatment. But still there is debate about the choice of sperm source in cryptozoospermic men candidate for ICSI. OBJE...

Intracytoplasmic sperm injection (ICSI) is an increasingly popular means of treating infertility in couples who wish to conceive. However, there are many potential complications that can be faced by the clinician while performing ICSI. These complications and other related issues are discussed, with an emphasis on understanding how these issues are being resolved, or how they can be resolved in...

Failure of male pronucleus formation has hampered the success of intracytoplasmic sperm injection (ICSI) in swamp buffalo. The aim of the present study was to improve male pronucleus formation by pretreating sperm with various chemicals before ICSI. In Experiments1 and 2, sperm were treated according to one of the following protocols: (1) 0.1% Triton-X 100 (TX) for 1 min, (2) 10 μM calcium iono...