Type strains and 62 clinical isolates of Prevotella intermedia and Prevotella nigrescens were typed with the use of genomic DNA fingerprints and rRNA gene probes. The strains were further serotyped with monoclonal antibodies and characterized with SDS-PAGE, enzymatic activities, hemolysis and hemagglutination and coaggregation with Streptococcus and Actinomyces spp. P. intermedia and P. nigresc...

The ability of Prevotella intermedia to bind type I collagen was investigated. A simple method in which bacterial cells were allowed to attach to collagen-coated microtitre plate wells was used to characterize the activity. All strains of P. intermedia tested, as well as those of the closely related species Prevotella nigrescens, showed a capacity to attach to the collagen film. Exponential-pha...

Background: Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals’ foods to hydrolyze phytate and increase absorption of phosphorus. Objectives: Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stabilit...

The objective of this research was to evaluate, in laboratory, the development of Lutzomyia intermedia and Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) larvae, vectors of leishmaniasis in Brazil, in the following diets: industrialized food for rabbits, dogs, hamsters and aquarium fishes, besides liver powder, cooked lettuce, wheat germ, beer yeast, oat, wheat bran and a diet deno...

Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) is an independent cardiovascular risk factor. We investigated the plasma levels of Lp-PLA(2) activity and mass as a function of plasma lipid levels, LDL subclass profile, and oxidative stress in patients with β-thalassemia. Thirty-five patients with β-thalassemia major (β-TM) and 25 patients with β-thalassemia intermedia (β-TI) participated ...

Prevotella intermedia, a major periodontal pathogen, is increasingly implicated in human respiratory tract and cystic fibrosis lung infections. Nevertheless, the specific mechanisms employed by this pathogen remain only partially characterized and poorly understood, largely due to its total lack of genetic accessibility. Here, using Single Molecule, Real-Time (SMRT) genome and methylome sequenc...

1. Biosynthesis in the lead-haematoxylin positive pars intermedia cells of Sarotherodon mossambicus was analysed by SDS and acid-urea electrophoresis and high pressure liquid chromatography. 2. Pulse and pulse-chase experiments revealed that a non-glycosylated 30K-precursor was processed into a number of smaller products. 3. Melanotropic and endorphin-like peptides appeared to be the final prod...

This study aimed to investigate the antibacterial and antibiofilm activities of essential oils Cuminum cyminum (CEO) Foeniculum vulgare (FEO) on clinically isolated Porphyromonas gingivalis (P. gingivalis) Prevotella intermedia intermedia). Subgingival plaque samples were collected from periodontal pockets (≥6 mm) patients with generalized severe periodontitis. Microbiological molecular tests p...

Nyssomyia intermedia and Nyssomyia neivai constitute a species complex associated with Leishmania transmission. The aim of this study was to analyse the ecological profiles of the Ny. intermedia and Ny. neivai populations in a sympatric area in the Brazilian savannah along the banks of the Velhas River. Captures were performed from July 2003-June 2005 in two distinct environments: a gallery for...

The prevalence of infection with intestinal spirochaetes in chickens in Western Australia was assessed by selective culture of faecal samples. Colonisation was common, with 35.1% of layer flocks and 53.3% of broiler breeder flocks being positive. Spirochaetes were recovered significantly more frequently from flocks with diarrhoea or reduced production than from clinically normal flocks. The gen...