Background: Viral outgrowth assay (VOA) is a widely used culture assay to measure the latent HIV-1 reservoir harboring replication-competent HIV-1 in resting CD4+ T cells in patients on HAART. However, the assay is costly, and both labor and resource intensive. To overcome some of these issues with the VOA, we designed an assay using ultra deep sequencing (UDS), which directly analyzes the numb...

Under intensive aeration (1.3 l/l min) the associated growth of Rhodotorula rubra GED2 and Lactobacillus casei subsp. casei in cheese whey ultrafiltrate (55 g lactose/l) proceeded effectively for both cultures with production of maximum carotenoids (12.4 mg/l culture fluid). For maximum amount of carotenoids synthesized in the cell, the yeast required more intensive aeration than the aeration n...

The effects of maturity degree and culture type on oligosaccharide content were studied in soybean seed, a rich source of non-digestible galactooligosaccharides (GOS). Therefore, two commercial cultivars of yellow soybeans (ripe seeds) and two of green soybeans (unripe seeds) were chosen. One yellow and one green soybean seed were from intensive culture, while one yellow and one green soybean s...

The research conducted at site 7 in Krzyż Wielkopolski has provided evidence of long-term occupation this area from the Late Palaeolithic (Sviderian) to modern era. Undoubtedly most intensive is related Early Mesolithic communities Preboreal and Boreal Periods. However, pottery fragments, flint tools, perhaps also belemnite artefacts, all associated with societies Corded Ware culture, prove Neo...

INTRODUCTION Bloodstream infection (BSI) is a leading cause of mortality in critically ill patients. The mortality directly attributable to BSI has been estimated to be around 16% and 40% in general hospital population and Intensive Care Unit (ICU) population, respectively. The detection rate of these infections increases with the number of blood samples obtained for culture. The newer continuo...