Biopolymeric patches show enormous potential for the regeneration of infarcted myocardium tissues. However, most them usually lack appropriate mechanical performance, stability in water, and important functionalities; instance, antioxidant activity. Protein nanofibrils, such as lysozyme nanofibrils (LNFs), are biocompatible nanostructures with excellent water insolubility, activity exploited to...

Vitreoscilla hemoglobin (VHb) is accumulated at high levels in both soluble and insoluble forms when expressed from its native promoter on a pUC19-derived plasmid in Escherichia coli. Examination by atomic absorption spectroscopy and electron paramagnetic resonance spectroscopy revealed that the insoluble form uniformly lacks the heme prosthetic group (apoVHb). The purified soluble form contain...

In this study, we examined human growth hormone (hGH)-induced changes in nonionic detergent solubility characteristics of its receptor (hGHR). Exposure of IM-9 cells to hGH caused a time- and concentration-dependent loss of immunoblottable detergent-extractable hGHRs and a corresponding accumulation of receptors in a detergent-insoluble pool. At 37°C, the loss of detergent-soluble and the accum...

Amsacrine analog is a novel chemotherapeutic agent that provides potentially broad antitumor activity when compared to traditional amsacrine. However, the major limitation of amsacrine analog is that it is highly lipophilic, making it nonconductive to intravenous administration. The aim of this study was to utilize solid lipid nanoparticles (SLN) to resolve the delivery problem and to investiga...

In another paper [1914] an account will be found of a new method for the estimation of aspartic and glutaminic acids in the hydrolytic products of proteins, depending upon the insolubility of their calcium salts in alcohol. In studying the influence of lime upon glutaminic acid in aqueous solution at higher temperatures it was found that the amino-nitrogen content had been considerably reduced....

Various anthelmintics belonging to the pharmacolog~cal group of benzimidazoles (oxibendazole, thiabendazole, albendazole, oxfendazole, flubendazole, mebendazole, parbendazole, fenbendazole and triclabendazole) were tested and compared for their In vitro and in vivo activity against an infection of rainbow trout Onchorhynchus myhss by Gyrodactylus. The trout were also observed for signs of toxic...

Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. However, lack of expression and insolubility remain significant challenges to the expression and crystallization of these proteins. The SSGCID program uses a wheat germ cell-free expression system as a rescue pathway for proteins that are either not expressed or ins...