Kidney glucose 6-phosphatase has been shown to catalyze inorganic pyrophosphatase and inorganic pyrophosphate-, cytidine diphosphate-, cytidine triphosphate-, and mannose 6-phosphate-glucose phosphotransferase reactions. The catalytic properties of phosphohydrolase and phosphotransferase activities of the kidney enzyme were found to be very similar to those of corresponding hepatic activities (...

A procedure is described for the determination of the following 19 metabolites in 200 mg gastrocnemius muscle from guinea pig: glucose 1-phosphate and 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, 3-phospho- and 2-phosphoglyceric acid, phosphoenolpyruvate, pyruvate, glycerol, glycerol 3-phosphate, AMP, ADP, ATP, inorganic p...

The product inhibition of potato (Solanum tuberosum) tuber pyrophosphate:fructose-6-phosphate phosphotransferase by inorganic pyrophosphate and inorganic phosphate has been studied. The binding of substrates for the forward (glycolytic) and the reverse (gluconeogenic) reaction is random order, and occurs with only weak competition between the substrate pair fructose-6-phosphate and pyrophosphat...

1. The effects of ATP, inorganic phosphate and citrate on the relationship between fructose 6-phosphate concentration and initial velocity of reaction has been investigated with a partially purified preparation of rat-heart phosphofructokinase. 2. At low concentrations of ATP (<80mum) rate curves for fructose 6-phosphate approximated to Michaelis-Menten kinetics. At higher ATP concentrations ra...

The bacterial C-P lyase pathway is responsible for the metabolism of unactivated organophosphonates under conditions of phosphate starvation. The cleavage of the C-P bond within ribose-1-methylphosphonate-5-phosphate to form methane and 5-phospho-ribose-1,2-cyclic phosphate (PRcP) is catalyzed by the radical SAM enzyme PhnJ. In Escherichia coli the cyclic phosphate product is hydrolyzed to ribo...

In the autotrophic bacterium, Thiobacillus thiooxidans, the oxidation of sulfur is coupled to transfers of phosphate from the medium to the cells. CO(2) fixation is coupled to transfers of inorganic phosphate from the cells to the medium and is dependent, in the absence of concomitant sulfur oxidation, upon the amount of phosphate previously taken up during sulfur oxidation. The energy reservoi...

The inhibitory/repressive effect of the final reaction product (inorganic o-phosphate) and the inducing effect of a substrate (β-glycerophosphate) on the phosphatase activity and synthesis in a salt lake sediment were studied. The determinations have been differentially carried out, for acid, neutral and alkaline phosphatase, and in reaction mixtures without buffering. The acid phosphatase acti...

In 19491 our laboratory described the binding of inorganic phosphate by a mitochondrial preparation of rabbit kidney. This binding was shown to be linked to electron transfer since reagents that were found to inhibit this process exerted a comparable effect on the binding Although the phosphate taken up by the mitochondrion estimated as inorganic phosphate, nonetheless, some of the properties o...

Feedstuffs of plant origin do not contain enough digestible phosphorus to meet the requirements for animal production. For this reason additional inorganic P is added to animal diets. Feedstuffs of plant and animal origin as well as inorganic phosphate sources contain various amounts of phosphate that is available for biochemical functions. Therefore most Nutritionists include a safety factor t...