We developed a DNA sequencing-based method to detect mutations in the genome of drug-resistant Mycobacterium tuberculosis. Drug resistance in M. tuberculosis is caused by mutations in restricted regions of the genome. Eight genome regions associated with drug resistance, including rpoB for rifampin (RIF), katG and the mabA (fabG1)-inhA promoter for isoniazid (INH), embB for ethambutol (EMB), pn...

Less than 30% of multidrug-resistant tuberculosis (MDR-TB) patients are currently diagnosed, due to laboratory constraints. Molecular diagnostics enable rapid and simplified diagnosis. Newer-version line probe assays have not been evaluated against the WHO-endorsed Hain GenoType MTBDRplus (referred to as Hain version 1 [V1]) for the rapid detection of rifampin (RIF) and isoniazid (INH) resistan...

In this study Isoniazid (INH) as one of the first line drugs in treatment of Tuberculosis was investigated to be loaded in Solid Lipid Nanoparticles (SLNs) for reducing hepatotoxicity as well as prolonging drug release. High shear homogenization method was performed to prepare INH SLNs. To compare biodistribution of INH before and after loading in SLNs, INH was labeled by Technetium 99 (Tc99) a...

SETTING The dual epidemics of HIV-TB including MDR-TB are major contributors to high morbidity and mortality rates in South Africa. Rifampicin (RIF) resistance is regarded as a proxy for MDR-TB. Currently available molecular assays have the advantage of rapidly detecting resistant strains of MTB, but the GeneXpert does not detect isoniazid (INH) resistance and the GenoTypeMTBDRplus(LPA) assay m...

Nine structural genes (furA, katG, inhA, kasA, Rv0340, iniB, iniA, iniC, and efpA) and two regulatory regions (the oxyR-ahpC intergenic region and the promoter of mabA-inhA) in 87 isoniazid (INH)-monoresistant and 50 INH-susceptible Mycobacterium tuberculosis isolates collected from five provinces of China were analyzed by sequencing. Eighty-two (94.3%) INH-resistant isolates had mutations in t...

India contributes to approximately one-third of total global tuberculosis (TB) and one-fourth all Multi-Drug Resistant TB (MDR-TB) burden respectively. The First National drug resistance survey (2014-16) showed MDRTB rates 6.19% overall 2.14% in newly diagnosed cases. With the above problem primary among cases, present study was planned find prevalence Isoniazid (INH) Rifampicin treatment naïve...

Purpose: The antimicrobial susceptibility patterns of 200 isolates of Mycobacterium tuberculosis, recovered from patients with pulmonary tuberculosis (PTB) in the Sudan, was determined against the first-line antituberculosis drugs. Methods: Isolates were recovered from smear-positive sputum samples and confirmed biochemically and by PCR. Drug resistance of the isolates to isoniazid (INH), rifam...

The detection of drug resistance in tuberculosis takes several weeks with conventional methods, and there is a high cost associated with the use of faster methods like commercial kits or molecular techniques. This work evaluates the nitrate reductase method (NRM), the MTT colorimetric assay and resazurin (RES) as alternative assays for the rapid detection of resistance to first line antitubercu...