We studied anti-influenza cytotoxicity by bulk peripheral blood mononuclear leukocyte (PBL) cultures derived from older, chronically ill volunteers undergoing vaccination. Vaccinees received either cold-recombinant, live-attenuated influenza A/Korea/1/82 (H3N2) virus intranasally or inactivated monovalent influenza A/Taiwan/1/86 (H1N1) subvirion vaccine intramuscularly. PBL were collected pre- ...

We developed a direct enzyme immunoassay [EIA; Enzygnost Influenza A(Ag) and Enzygnost Influenza B(Ag)] for the direct detection of influenza A and B virus antigens in nasopharyngeal secretion specimens (NPS). The test is performed without sonification of specimens, and results are obtained within 4 h. A direct comparison between direct EIA and quantitation of virus shedding for influenza A and...

Influenza A virus is the respiratory pathogen responsible for influenza. Infection by the 2009 pandemic influenza A (H1N1) virus caused severe lower airway inflammation and pneumonia. Asthma is a chronic inflammatory disorder of the airways that affects the entire brachial tree, and was one of the commonest underlying medical conditions among patients hospitalized with the 2009 pandemic influen...

the serious influenza-associated complications among immunodeficient individuals such as those who are infected with human immunodeficiency virus (hiv), highlights the importance of influenza vaccination in these people. therefore, the current study aimed to investigate the antibody responses to influenza vaccine in this group. two hundred subjects were recruited, during autumn 2010 and 2011, t...

Background and Aims: Avian Influenza (AI) H9N2 subtype was first reported to infect turkeys in the United States in 1966 and has been panzootic in Eurasia. In Iran, the H9N2 virus was first isolated from broiler chickens in 1998 in Ghazvin province and it is the most prevalent subtype of influenza virus in poultry industry in Iran at the present time. Materials and Methods: In this study, we se...

UNLABELLED Influenza B virus causes annual epidemics and, along with influenza A virus, accounts for substantial disease and economic burden throughout the world. Influenza B virus infects only humans and some marine mammals and is not responsible for pandemics, possibly due to a very low frequency of reassortment and a lower evolutionary rate than that of influenza A virus. Influenza B virus h...

Swine influenza virus structure Influenza viruses are enveloped, segmented, single-stranded, negative-sense RNA viruses belonging to the family Orthomyxoviridae.2 The Orthomyxoviridae family consists of four genera – influenza A virus, influenza B virus, influenza C virus, and thogotovirus.2 As influenza A viruses cause disease in animals, birds, and humans,3 including swine, this genus will be...

results 111 female and 142 male were available from a total of 253 confirmed h1n1 cases. the minimum and maximum ages were 1.5 and 90 years, respectively. the most common symptoms were fever and cough. 144 cases had record of hospitalization, 100 cases were undergone outpatient treatment and nine cases have not recorded the treatment status. the most frequent underlying manifestation was hypert...

Background and Aims: The novel approaches in influenza vaccination have targeted more conserved viral proteins such as nucleoprotein (NP) to provide cross protection against all serotypes of influenza A viruses. Influenza specific cytotoxic T lymphocytes (CTL) are able to lyse influenza-infected cells by recognition of NP, the major target molecule in virus for CTL responses. On the other hand,...

UNLABELLED Secondary Streptococcus pneumoniae infection after influenza is a significant clinical complication resulting in morbidity and sometimes mortality. Prior influenza virus infection has been demonstrated to impair the macrophage and neutrophil response to the subsequent pneumococcal infection. In contrast, how a secondary pneumococcal infection after influenza can affect the adaptive i...