BACKGROUND Parasitic nematodes cause animal and human diseases of major socio-economic importance worldwide. The suppression of parasite development at particular developmental stages could provide an alternative approach for nematode control. In this study, Ascaris suum was used as a model system in the study of the differentially expressed genes in the infective L3 stage. METHODS The gene (...

The current control strategies employing chemotherapy with diethylcarbamazine, ivermectin and albendazole have reduced transmission in some filaria-endemic areas, there is growing interest for complementary approaches, such as vaccines especially in light of threat of parasite developing resistance to mainstay drugs. We earlier demonstrated recombinant heavy chain myosin of B. malayi (Bm-Myo) a...

Latar Belakang: Cendawan nematofagus merupakan cendawan tanah yang dapat mereduksi cacing nematoda, ditemukan di berbagai tempat dan terbukti efektif sebagai agen biocontrol, tergolong seperti: Arthrobotrys spp., Catenaria anguillulae, Drechmeria Duddingtonia flagrans., Geniculifera Harposporium Monacrosporium Nematocnus Pleurotus Tridentaria implicans, Verticillium balanoides. Tujuan: mengetah...

Third-stage infective larvae (L3i) of Brugia malayi are developmentally arrested in mosquitoes but must quickly adapt to a new environment when they enter mammalian hosts to initiate infections. These changes can be studied by in vitro culture of L3 (L3c) under conditions that permit molting of L3-L4. Irradiated L3 (L3ir) have stunted growth and limited lifespan in mammalian hosts, and they ind...

To evaluate the impact of onchocerciasis control programmes on the annual transmission potential, it is customary to monitor the man-biting rate of vector Siniuliuin (Diptera: Simuliidae) and the number of Oizclzocerca (Nematoda: Onchocercidae) infective larvae found in them (Walsh et al., 1978). In addition to the transmission of Onchocerca volvulus (Leuckart) causing human onchocerciasis, Sim...

A 2-step roller culture system was designed that supported optimally the development of late-thirdstage larvae (L3) of Ascaris suum, obtained from rabbit lungs, to mature adults in vitro. The culture system consisted of 1) medium API-18 for 7 days, and 2) thereafter, medium API-1 supplemented with bovine hemin. Cultures were gassed with 85% nitrogen/5% oxygen/10% carbon dioxide and incubated at...

A study was undertaken to elucidate the impact of an undescribed Nosema sp. on the southwestern corn borer (SWCB; Diatraea grandiosella Dyar). The Nosema sp. (isolate 506) included in the study was isolated from an overwintering SWCB larva in Mississippi. It was highly infectious per os, with a median infective dose of 2.0 x 10(3) spores per larva. Even at the highest dosage tested (10(7) spore...