A study was conducted to determine the kinetics of Fasciola gigantica infection induced antibody response against oxyclozanidelevamisole in naturally infected bovine of South Gujarat, India. Faecal analysis on before, 1st, 4th, 8th, 11th and 37th days post treatment (DPT) recorded 67.14, 34.29, 5.71, 0 and 0% parasites infected bovines. Either parasite(s) positive indigenous cattle or buffalo b...

The current study was carried out to determine the sero-prevalence and molecular detection of Brucella species in persons involved in pork production in Punjab state of India. The sample size was selected using survey toolbox and a total of 123 blood samples were collected from pig farmers and slaughter house workers. The serum samples were tested using Rose Bengal Plate Test (RBPT), Standard T...

Regarding harmful agents in livestock, parasites play an important role moreover it also effect 1/4th of the human populace by various zoonotic diseases (e.g. Toxplasma, Cryptosporidium, Trypanosomiasis, etc.). In livestock the parasites successful invade host immune responses, so early diagnosis of parasitic agents by various immunodiagnostic techniques is very important. Numerous immunologica...

Objectives: Progressive multifocal leukoencephalopathy (PML) is caused by a reactivated infection with the human polyomavirus JC (JCV). Primary infection with JCV has not been linked to any disease in humans. Using recombinant viral protein 1 (VP1) of JCV as antigen, we developed an enzyme linked immunosorbent assay (ELISA) for the detection of VP1-speciŽc IgM. Methods: Two different direct ELI...

We compared washed nasal epithelial cells with unfractionated nasal secretions as sources of respiratory syncytial virus (RSV) antigens in an indirect enzyme-linked immunosorbent assay (ELISA). Of 28 infants positive for RSV by virus isolation or direct immunofluorescence or both, 27 (96%) were positive by ELISA with whole nasal secretions, whereas only 19 (68%) were positive by ELISA with the ...

Several protocols were developed and evaluated for the capture of foot-and-mouth disease (FMD) virus antigen on a microtitre plate to enable RNA extraction and reverse transcription to be carried out in the plate wells prior to PCR amplification of the cDNA product. One protocol enabled the FMD viral antigen from suspensions of clinical samples to be captured in the well by coating with a cockt...

In this study we report a new concept of printing low-cost two-dimensional micro-zone plates for chemical and biochemical assays and ELISA. It is different from the concept of forming multi-zone micro-zones on paper by hydrophobic barriers first reported by Yagoda. Instead, the desired multi-zone micro-zone pattern was first printed using a UV-curable varnish onto a polymer film; then fine powd...

Background: Expression of virus coat protein (CP) in Escherichia coli often leads to production of partially folded aggregated proteins which are called inclusion bodies. Grapevine fanleaf virus (GFLV) is one of the most serious and widespread grapevine virus diseases around the world and in Iran.Objective: The main objective of this study was to find a s...